Recombinant Serine protease espP (espP), partial

中文名稱：

大腸桿菌O157：H7espP重組蛋白
貨號：

CSB-YP765862EOD
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌O157：H7espP重組蛋白
貨號：

CSB-EP765862EOD
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌O157：H7espP重組蛋白
貨號：

CSB-EP765862EOD-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌O157：H7espP重組蛋白
貨號：

CSB-BP765862EOD
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌O157：H7espP重組蛋白
貨號：

CSB-MP765862EOD
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

espP
Uniprot No.：

Q7BSW5
別名：

espP; L7020; ECO57PM78Serine protease EspP; EC 3.4.21.-) [Cleaved into: Secreted autotransporter protein EspP; Extracellular serine protease plasmid-encoded EspP); Autotransporter protein EspP translocator]
種屬：

Escherichia coli O157:H7
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.
基因功能參考文獻：
1. Serine protease EspP from enterohemorrhagic Escherichia coli is sufficient to induce shiga toxin macropinocytosis in intestinal epithelium. PMID: 23874912
2. Point mutations introduced at specific locations near the middle or N terminus of the EspP beta-helix that perturb folding also impair secretion, but to a lesser degree. PMID: 24165126
3. Data show the completion of EspP beta domain assembly is regulated by an intrinsic checkpoint mechanism that requires the completion of passenger domain secretion. PMID: 21646511
4. Efficient secretion of the passenger domain of the Escherichia coli O157:H7 autotransporter EspP requires the stable folding of a C-terminal approximately 17-kDa passenger domain segment. PMID: 20876094
5. Adherence of E. coli O157:H7 was significantly impaired by espP mutation but restored upon addition of highly purified exogenous EspP. PMID: 17451446
6. The data suggest that the EspP beta-domain and an embedded polypeptide segment are integrated into the outer membrane as a single pre-formed unit. PMID: 18047580
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亞細胞定位：

[Serine protease EspP]: Periplasm.; [Secreted autotransporter protein EspP]: Secreted. Cell surface.; [Autotransporter protein EspP translocator]: Cell outer membrane; Multi-pass membrane protein.
數據庫鏈接：

KEGG: ece:Z_L7020

STRING: 155864.L7020