Recombinant Human R-spondin-1 (RSPO1), partial (Active)

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中文名稱：

人RSPO1重組蛋白
品名簡稱：

Recombinant Human RSPO1 protein, partial (Active)
貨號：

CSB-MP644834HU1
說明書：

MSDS Datasheet
規格：

￥936
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human RSPO1 at 2 μg/ml can bind Human LGR5(CSB-MP012906HU1), the EC₅₀ is 124.0-174.1 ng/mL. Biological Activity Assay
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靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA. Immobilized Human RSPO1 at 2 μg/mL can bind Human LGR5(CSB-MP012906HU1), the EC₅₀ is 124.0-174.1 ng/mL.
基因名：

RSPO1
Uniprot No.：

Q2MKA7
別名：

(Roof plate-specific spondin-1) (hRspo1)
分子結構：
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

30.2 kDa
表達區域：

31-263aa
氨基酸序列

RISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA
蛋白標簽：

C-terminal 10xHis-Avi-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The recombinant human R-spondin-1 (RSPO1) protein was produced in the mammalian cells with the plasmid vector containing a synthesized gene. The synthesized gene was formed by fusing the 10xHis-Avi-tag gene to the C-terminus of the gene encoding the 31-263aa of the human RSPO1. This recombinant RSPO1 protein has been validated to be biologically active. In the functional ELISA, this human RSPO1 can bind to the human LGR5, with the EC₅₀ of 124.0-174.1 ng/mL. Its purity is greater than 95% estimated by SDS-PAGE, and its endotoxin level is less than 1.0 EU/ug assessed by the LAL method.

In bipotent gonads during embryonic development, RSPO1 functions as an early ovarian determinant to activate the WNT/β-catenin signaling pathway, with WNT4 being the key ligand, thus allowing ovarian differentiation. In male gonads, Sry binds to the β-catenin/TCF transcription complex and inhibits the activation of WNT signaling by RSPO1, allowing testis differentiation. RSPO1 also can promote osteoblastic differentiation and bone formation, protect against inflammatory bone damage, and reduce age-induced bone loss. RSPO1 is involved in the pathogenesis of De la Chapelle syndrome, true hermaphroditism (TH), and intestinal ischemia-reperfusion injury (IRI). Understanding how RSPO1 participates in sexual differentiation will lead to greater breakthroughs and provide more help for clinical diagnosis and treatment.
Datasheet & COA：

Please contact us to get it.

產品評價

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5.0分 - 1 個評價

實驗應用：結合實驗/蛋白間互作

產品評價：我使用CSB-MP644834HU1產品用于蛋白篩選，利用SPR對候選蛋白親和力強弱進行篩選，實驗結果不錯，準備申報專利。證明CUSABIO的蛋白產品質量確實不錯！值得推薦！

By 鄧老師

靶點詳情

功能：

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1.
基因功能參考文獻：
1. first report of a RSPO1 missense mutation in association with human disease (familial 46,XX disorder of sexual development) . PMID: 29262419
2. Rspo1 increases the number of Lgr5(+) liver stem cells in human liver fibrosis tissues, and once they are isolated, these cells are able to form organoids, and treatment with HGF/Rspo1 promotes their expansion PMID: 29079780
3. RSPOs facilitate HSC activation and promote liver fibrogenesis by enhancing the Wnt pathway PMID: 27572318
4. Role of RSPO1 in gastric cancer PMID: 28219935
5. Results showed that Rspo1 expression is downregulated in adult follicles but its activation is sufficient in promoting ovarian tumors supporting its direct involvement in the initiation of ovarian cancers. PMID: 27270435
6. We highlight the cooperation of WNT4, RSPO1 and FOXL2 within a regulatory network and the need for further research to better understand their role in defining and maintaining ovarian identity. PMID: 27604691
7. Rspo1 glycosylation at Asn137 is essential for secretion and stability but not for heparin binding. PMID: 27314333
8. DPY19L3-mediated C-mannosylation of Rspo1 at tryptophan(156) is required for Rspo1 secretion. PMID: 26764097
9. Genetic variants in ZC3H11B, RSPO1, and GJD2 are associated with susceptibility to the development of high myopia in a Han Chinese population. PMID: 26485405
10. R-Spondin1 has an effect on radiosensitivity of glioma cells PMID: 25865226
11. ZNRF3 and LGR4-binding sites in RSPO1 are required for Wnt signaling. PMID: 24165923
12. Changes in the expression levels of IRS1, IRS2, RIPK2, RSPO1, and DNA JC15 genes might contribute to the development of insulin resistance and glucose intolerance in the obese boys. PMID: 26040030
13. the LGR4-Rspo1 complex crystal structure shows divergent mechanisms of ligand recognition by leucine-rich repeat G-protein-coupled receptors PMID: 25480784
14. ZNRF3 binds RSPO1 and LGR5-RSPO1 with micromolar affinity via RSPO1 furin-like 1 (Fu1) domain. PMID: 24349440
15. overexpressed in fibrotic liver tissue PMID: 25218283
16. Loss of RSPO1 expression is associated with invasive ductal carcinoma of the breast. PMID: 24373193
17. RSPO-LGR4 not only induces the clearance of RNF43/ZNRF3 to increase Wnt receptor levels but also recruits IQGAP1 into the Wnt signaling complex. PMID: 24639526
18. Crystal structures of the Lgr4 ectodomain alone and bound to Rspo1. PMID: 23891289
19. In conclusion, present study highlights the role of Rspo 1 in bone remodeling where it activates Wnt signaling to induce differentiation, as shown in human as well murine in vitro osteoblast cell models. PMID: 23617070
20. Recent developments have demonstrated that ovarian development is an active process (rather than a default process); ovarian development/function requires expression of RSPO1, WNT4, and FOXL2. [REVIEW] PMID: 23044875
21. R-Spondin potentiates Wnt/beta-catenin signaling through orphan receptors LGR4 and LGR5 PMID: 22815884
22. study provides new mechanistic insights into the regulation of Wnt receptor turnover, and reveals ZNRF3 as a tractable target for therapeutic exploration PMID: 22575959
23. R-spondin1 is upregulated during critical stages of early human ovary development and may function as a tissue-specific amplifier of beta-catenin signaling to oppose testis determination. PMID: 21297984
24. identified a gene, R-spondin1, with potent and specific proliferative effects on intestinal crypt cells PMID: 16109882
25. Human R-spondin1 (RSPO1) is the gene disrupted in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. PMID: 17041600
26. RSPO1 regulates Wnt signaling by inhibiting internalization of LRP6. PMID: 17804805
27. Mutational analysis of the RSPO1 gene in a 46,XX woman with true hermaphroditism, palmoplantar keratoderma, congenital bilateral corneal opacities, onychodystrophy, and hearing impairment, is reported. PMID: 18085567
28. R-sponin1 (Rspo1) acted synergistically with Wnt3A to activate Wnt/beta-catenin signaling in the uncommitted mesenchymal C2C12 cells. PMID: 18242177
29. SRY represses the transcriptional of the Rspo1/Wnt target genes involved in ovarian determination. PMID: 19376480
30. Mice treated with R-spondin1 showed increased intestinal epithelial healing, providing a protective effect against chemotherapy-induced intestinal mucositis. PMID: 16306530
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相關疾病：

Keratoderma, palmoplantar, with squamous cell carcinoma of skin and sex reversal (PKKSCC)
亞細胞定位：

Secreted. Nucleus.
蛋白家族：

R-spondin family
組織特異性：

Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.
數據庫鏈接：

HGNC: 21679

OMIM: 609595

KEGG: hsa:284654

STRING: 9606.ENSP00000348944

UniGene: Hs.135015