Recombinant Rat Agrin (Agrn), partial

中文名稱：

Recombinant Rat Agrin(Agrn) ,partial
貨號(hào)：

CSB-YP001461RA
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rat Agrin(Agrn) ,partial
貨號(hào)：

CSB-EP001461RA
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rat Agrin(Agrn) ,partial
貨號(hào)：

CSB-EP001461RA-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rat Agrin(Agrn) ,partial
貨號(hào)：

CSB-BP001461RA
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rat Agrin(Agrn) ,partial
貨號(hào)：

CSB-MP001461RA
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Agrn
Uniprot No.：

P25304
別名：

Agrn; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment; C90); Agrin C-terminal 22 kDa fragment; C22)]
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.; Isoform 1, isoform 4, isoform 5 and isoform 6: neuron-specific (z+) isoforms that contain C-terminal insertions of 8-19 AA are potent activators of AChR clustering. Isoform 5, agrin (z+8), containing the 8-AA insert, forms a receptor complex in myotubules containing the neuronal AGRN, the muscle-specific kinase MUSK and LRP4, a member of the LDL receptor family. The splicing factors, NOVA1 and NOVA2, regulate AGRN splicing and production of the 'z' isoforms.; is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling.; this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.
基因功能參考文獻(xiàn)：
1. compared expression of agrin isoforms with the expression of AChE-T and ColQ in the developing spinal cord at the stages before and after the formation of NMJ basal lamina PMID: 23117006
2. present the first crystal structure of an agrin-LRP4 complex which is essential for neuronal agrin-induced acetylcholine receptor (AChR) clustering PMID: 22302937
3. agrin modulates neuropathic pain through NR1 phosphorylation in GABA neurons PMID: 21068333
4. a model wherein the z8 insert is a Ca(2+)-responsive allosteric element that is essential in forming an active conformation in neuronal a PMID: 20566625
5. Agrin binds BMP2, BMP4 and TGFbeta1 with relatively high affinity. PMID: 20505824
6. different regions within the agrin protein are responsible for synapse formation at the neuromuscular junction and for process formation in central nervous system neurons PMID: 19940118
7. The expression of agrin was studied during muscle development in a novel three-dimensional muscle-neuronal coculture system. PMID: 12955494
8. Agrin deletion mutants show that axonal targeting of agrin depends on multiple domains that function in an additive fashion, including the very N-terminal portions and the C-terminal half of the molecule. PMID: 14697677
9. Agrn becomes concentrated at neuroeffector and neuromuscular juncions in developing urinary bladder. PMID: 15711988
10. Agrin may play a pivotal role in synaptogenesis in promoting a rapid switch between electrical coupling and synaptic neurotransmission. PMID: 15883200
11. Agrin plays a role as a regulator of intercellular communication particularly junctional electric coupling and synapatic remodeling. PMID: 16274639
12. Results suggest that by positively regulating filopodia in developing neurons, transmembrane agrin may influence the pattern of neurite outgrowth and synapse formation. PMID: 16860570
13. Agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and due to modulation of RyRs and L-type Ca(2+) channels. A role for neural agrin in skeletal muscle maturation is proposed. PMID: 18003748
14. Agrin plays an important role in the sprouting phase of reactive synaptogenesis; both its expression and distribution are correlated with extent of successful recovery after TBI. PMID: 18627255
15. increasing the concentration of an ECM molecule, i.e. agrin, may enhance nicotine effects on pCREB PMID: 18639611
16. transient post-ischemic hypothermia stabilizes brain vessels and reduces blood brain barrier disruption in part by preventing proteolytic degradation of regulatory basement membrane constituents, SPARC and agrin PMID: 19285050
17. The results suggest a physiological role for Tm-agrin in the maturation of hippocampal neurons involving positive regulation of dendritic filopodia and promotion of synaptogenesis, but also suggest a role for axonal agrin in synaptogenesis. PMID: 19524020
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亞細(xì)胞定位：

Cell junction, synapse. Cell membrane; Single-pass type II membrane protein.
組織特異性：

Embryonic nervous system and muscle.
數(shù)據(jù)庫鏈接：

KEGG: rno:25592

STRING: 10116.ENSRNOP00000050623

UniGene: Rn.2163