Mouse Immunoglobulin M,IgM ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠免疫球蛋白M(IgM)酶聯免疫試劑盒（CSB-E07977m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgM含量。IgM是人體內重要抗體，在機體早期免疫防御中發揮關鍵作用。其背景在于能高效結合抗原并激活補體系統。研究機制主要聚焦于其結構與功能關系，以及和疾病發生發展的關聯，探索以其為靶點開發免疫治療藥物。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，該產品適用于感染性疾病模型研究、疫苗免疫原性評估、免疫缺陷動物模型構建等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgM
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

62.5 ng/mL-4000 ng/mL
靈敏度：

62.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	93
	Range %	88-94
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	89
	Range %	85-94
1:8000	Average %	97
	Range %	91-101

回收率：

The recovery of mouse IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	103	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.