Rabbit Immunoglobulin M,IgM ELISA Kit

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產品詳情

產品描述：

兔子免疫球蛋白M(IgM)酶聯免疫試劑盒（CSB-E06950Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgM含量。IgM是人體免疫系統中重要的免疫球蛋白。在它是個體發育過程中最早合成和分泌的抗體。其研究機制主要聚焦在免疫識別與防御，可快速結合病原體激活補體系統，在抗感染初期發揮關鍵作用，也在自身免疫病等方面有重要研究價值。試劑盒檢測范圍為15.6 ng/mL-4000 ng/mL，在評估實驗動物免疫狀態或感染模型建立等科研場景中廣泛應用；適用于疫苗開發、感染性疾病動物模型研究、免疫抑制劑效果評估等科研領域本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgM
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

15.6 ng/mL-4000 ng/mL
靈敏度：

4 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	87-105
1:2000	Average %	92
	Range %	85-100
1:4000	Average %	93
	Range %	89-100
1:8000	Average %	94
	Range %	85-99

回收率：

The recovery of rabbit IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.