Mouse Homocysteic acid,Hcy ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠同型半胱氨酸(Hcy)酶聯(lián)免疫試劑盒（CSB-E08897m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Hcy含量。同型半胱氨酸（Hcy）是蛋氨酸代謝的中間產(chǎn)物。高Hcy水平是心血管疾病、神經(jīng)系統(tǒng)疾病等多種疾病的獨立危險因素。其研究機(jī)制主要圍繞Hcy誘導(dǎo)氧化應(yīng)激、損傷血管內(nèi)皮細(xì)胞、影響細(xì)胞甲基化反應(yīng)等方面，以揭示其在疾病發(fā)生發(fā)展中的作用。試劑盒檢測范圍為15.6 pmol/mL-1000 pmol/mL，適用于科研場景中探究Hcy在心血管疾病模型、神經(jīng)損傷研究或營養(yǎng)代謝實驗中的動態(tài)變化，也可用于藥物干預(yù)或基因修飾后Hcy代謝通路的機(jī)制研究，為相關(guān)疾病模型構(gòu)建及代謝調(diào)控分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

Hcy
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

15.6 pmol/mL-1000 pmol/mL
靈敏度：

3.9 pmol/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	98
	Range %	93-102
1:40	Average %	100
	Range %	95-105
1:80	Average %	97
	Range %	91-103
1:160	Average %	93
	Range %	88-99

回收率：

The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	99	96-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.