Human Homocysteic acid,Hcy ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人同型半胱氨酸(Hcy)酶聯(lián)免疫試劑盒（CSB-E08895h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、尿液樣本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代謝中間產(chǎn)物。其水平異常升高被視為心腦血管疾病等的獨立危險因素。它會損傷血管內(nèi)皮細胞，促進血栓形成，影響脂質(zhì)代謝，還能引發(fā)氧化應(yīng)激反應(yīng)，干擾正常細胞功能。試劑盒檢測范圍為0.78 nmol/mL-50 nmol/mL，本產(chǎn)品適用于疾病分子機制研究、營養(yǎng)代謝分析、藥物干預(yù)效果評估及生物標(biāo)志物篩查等科研場景，為實驗室提供標(biāo)準(zhǔn)化、高重復(fù)性的Hcy檢測方案，僅限科學(xué)研究使用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

Hcy
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, urine
檢測范圍：

0.78 nmol/mL-50 nmol/mL
靈敏度：

0.195 nmol/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HCY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	98
	Range %	91-105
1:4	Average %	89
	Range %	84-96
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human HCY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	92	89-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.