Human Afamin(AFM) ELISA kit

Human Afamin(AFM) ELISA kit

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中文名稱：

人Afamin(AFM)酶聯免疫試劑盒
貨號：

CSB-EL001419HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

人Afamin(AFM)酶聯免疫試劑盒（CSB-EL001419HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AFM含量。試劑盒檢測范圍為3.9 ng/mL-250 ng/mL，適用于科研領域對AFM在代謝調控、肝臟功能評估及腫瘤微環境等方向的研究；為探索AFM在脂質代謝異常、卵巢癌生物標志物篩選或慢性炎癥相關疾病中的分子機制提供可靠工具，尤其適用于基礎醫學研究、疾病模型構建及藥物干預效果評價等場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

AFAM_HUMAN ELISA Kit; Afamin ELISA Kit; Afm ELISA Kit; ALB 2 ELISA Kit; ALB2 ELISA Kit; ALBA ELISA Kit; ALF ELISA Kit; Alpha albumin ELISA Kit; Alpha-Alb ELISA Kit; Alpha-albumin ELISA Kit
縮寫：

AFM
Uniprot No.：

P43652
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.9 ng/mL-250 ng/mL
靈敏度：

0.97 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	90-102
1:2000	Average %	89
	Range %	84-95
1:4000	Average %	100
	Range %	95-106
1:8000	Average %	97
	Range %	92-104

回收率：

The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	102	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
250	2.024	2.098	2.061	1.929
125	1.715	1.761	1.738	1.606
62.5	1.334	1.364	1.349	1.217
31.25	0.893	0.884	0.889	0.757
15.6	0.567	0.546	0.557	0.425
7.8	0.321	0.349	0.335	0.203
3.9	0.261	0.246	0.254	0.122
0	0.131	0.133	0.132

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Fetuin B alleviates testosterone propionate-induced oxidative stress and mitochondrial dysfunction in KGN cells by upregulating the TGFR2/SMAD3 pathway Y Gao,ResearchSquare,2022
Seminal Plasma and Serum Afamin Levels Are Associated with Infertility in Men with Oligoasthenoteratozoospermia R Nu?ez-Calonge,Reproductive Sciences,2021
Use of liquid chromatography-tandem mass spectrometry to perform urinary proteomic analysis of children with IgA nephropathy and Henoch-Sch?nlein purpura nephritis X Fang,Journal of Proteomics,2020

功能：

Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.
基因功能參考文獻：
1. The results showed significantly higher serum afamin levels in women with pre-eclampsia and gestational diabetes mellitus compared to healthy pregnant women. PMID: 29405972
2. LDL apheresis moderately decreases the circulating levels of afamin parallel to lowering HDL-C and ApoA1 levels. PMID: 30247793
3. The concentration of afamin decreases with the severity of alcoholic liver cirrhosis. PMID: 30260179
4. Afamin binds palmitoylated serine 209 of Wnt3a, providing a structural basis how afamin solubilizes hydrophobic and poorly soluble Wnt proteins. PMID: 29153507
5. Results provide evidence that afamin could promote glycometabolism by up-regulating the glucose metabolism key enzymes in papillary thyroid carcinoma. PMID: 27329154
6. Data suggest that up-regulation of AFM levels in serum in women with polycystic ovary syndrome can be used as a biological markers of those women with increased risk of developing metabolic syndrome, especially those with insulin resistance. PMID: 25208973
7. A linear increase in afamin during pregnancy may serve as basic reference for subsequent investigations of afamin in pregnancy-related disorders. PMID: 24768783
8. Afamin levels were altered significantly in the peritoneal fluid of women with endometriosis compared with disease-free controls. PMID: 20858448
9. Binding of bilirubin with human serum albumin was studied by absorption, fluorescence and CD spectroscopy PMID: 12063119
10. The protein structure of afamin has been determined by homology modeling and its in vitro binding properties confirmed by docking calculations on the proposed structure. PMID: 12463752
11. vitamin E-binding properties were confirmed; found abundant presence of this protein in plasma, extravascular fluids such as follicular, and cerebrospinal fluids PMID: 15952736
12. afamin might be a new family member of binding/transport proteins contributing to alpha-tocopherol homeostasis at the blood-brain barrier PMID: 19046407
13. Reduced Afamin expression is associated with ovarian cancer. PMID: 19336561
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亞細胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
組織特異性：

High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).
數據庫鏈接：

HGNC: 316

OMIM: 104145

KEGG: hsa:173

STRING: 9606.ENSP00000226355

UniGene: Hs.168718

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.