Dog matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬基質金屬蛋白酶9/明膠酶B(MMP-9/Gelatinase B)酶聯免疫試劑盒（CSB-E13887c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的MMP9含量。MMP9 即基質金屬蛋白酶 9，在細胞外基質降解等過程中發揮重要作用。其異常表達與腫瘤侵襲轉移、炎癥反應、心血管疾病等密切相關。研究機制涉及調控其表達與活性，探索相關信號通路，以開發針對 MMP9 的治療策略。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，的應用場景包括但不限于犬類炎癥性疾病模型研究、腫瘤微環境分析、創傷愈合機制探索等基礎科研領域，為研究者提供可靠的蛋白表達數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
縮寫：

MMP9
Uniprot No.：

O18733
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-105
1:2	Average %	88
	Range %	80-96
1:4	Average %	87
	Range %	85-90
1:8	Average %	92
	Range %	88-107

回收率：

The recovery of dog MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	97	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

STRING: 9615.ENSCAFP00000035167

UniGene: Cfa.3470