ALPI
堿性磷酸酶（ALP或AKP、AP）是廣泛分布于人體肝臟、骨骼、腸、腎和胎盤等組織的單磷脂水解酶。這種酶能催化核酸分子脫掉5’磷酸基團，從而使DNA或RNA片段的5’-P末端轉換成5’-OH末端。
本身是一種膜結合金屬糖蛋白，由兩個亞單位組成，作為同源二聚體酶，包括四種同工酶，分別是組織非特異性AP(TNAP)、腸型AP(IAP)、胎盤型AP(PLAP)和類PLAP。
TNAP主要在肝臟、腎臟和骨組織中表達，即肝/骨/腎型AP。其中骨型AP是成骨細胞的一種細胞外酶，能被釋放到血液循環中，在機體的生理功能主要是在成骨過程中水解磷酸酯，為羥磷灰石的沉積提供必需的磷酸，同時水解焦磷酸鹽，解除其對骨鹽形成的抑制作用，有利于成骨過程。
IAP、PLAP和類PLAP為組織特異性AKP。IAP是一種能水解單磷酸酯的刷狀緣蛋白,可進一步分為胎兒IAP和成人IAP。該蛋白由腸黏膜上皮微絨毛、腎近曲小管遠側端和子宮分泌，胎兒IAP又稱笠原同工酶，它作為腫瘤標志物，出現于肝癌及其它器官發生腫瘤的病人血清中。PLAP主要由孕8周后的胎盤合胞體滋養層細胞微絨毛分泌，肺臟、卵巢、子官、胃腸道及其它臟器也分泌少量的PLAP，PLAP在人體的生理功能主要是參與細胞膜對物質的主動運輸及鈣、磷代謝，提供胎兒生長發育所需營養，對胎兒的生長發育起著重要作用，動態觀察胎盤型堿性磷酸酶活性可作為妊娠的一項指標。類PLAP又稱Nagao同工酶，本質是精原細胞AP(GCAP)，是一種腫瘤標記物，出現于多種癌癥中，如精原細胞瘤、卵巢癌等。
熱銷產品
● Recombinant Rat Intestinal-type alkaline phosphatase 1(Alpi) (Active) (CSB-MP001627RA)
驗證數據
Greater than 95% as determined by SDS-PAGE.
Less than 1.0 EU/ug as determined by LAL method.
Unit Definition: One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate (pNPP), in 1 minute at 37°C, pH10.0.The specific activity is <10370.37 U/mg.
● Recombinant Human Intestinal-type alkaline phosphatase(ALPI) (Active) (CSB-MP001627HU)
驗證數據
Greater than 95% as determined by SDS-PAGE.
Less than 1.0 EU/ug as determined by LAL method.
Unit Definition: One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate (pNPP), in 1 minute at 37°C, pH10.0. The specific activity is < 8836.463 U/mg.
ALPI Antibodies
ALPI for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA11989B0Rb | ALPI Antibody, HRP conjugated | Human | ELISA |
| CSB-PA11989A0Rb | ALPI Antibody | Human | ELISA, WB |
| CSB-PA921124 | ALPI Antibody | Human | ELISA,IHC |
ALPI Proteins
ALPI Proteins for Bos taurus (Bovine)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP001627BO CSB-EP001627BO CSB-BP001627BO CSB-MP001627BO CSB-EP001627BO-B |
Recombinant Bovine Intestinal-type alkaline phosphatase (ALPI) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ALPI Proteins for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-EP001627RA CSB-BP001627RA CSB-EP001627RA-B |
Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) | E.coli Baculovirus In Vivo Biotinylation in E.coli |
| CSB-YP001627RA | Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) | Yeast |
| CSB-MP001627RA | Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) (Active) | Mammalian cell |
ALPI Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP001627HU | Recombinant Human Intestinal-type alkaline phosphatase (ALPI) | Yeast |
| CSB-EP001627HU1 | Recombinant Human Intestinal-type alkaline phosphatase (ALPI), partial | E.coli |
| CSB-BP001627HU CSB-EP001627HU-B |
Recombinant Human Intestinal-type alkaline phosphatase (ALPI) | Baculovirus In Vivo Biotinylation in E.coli |
| CSB-EP001627HU | Recombinant Human Intestinal-type alkaline phosphatase (ALPI) | E.coli |
| CSB-MP001627HU | Recombinant Human Intestinal-type alkaline phosphatase (ALPI) (Active) | Mammalian cell |
ALPI ELISA Kit
ALPI ELISA Kit for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-E09709h | Human Intestinal-type alkaline phosphatase,ALPI ELISA Kit | serum, plasma, tissue homogenates | 0.78 ng/mL |
