Recombinant Rat Copper-transporting ATPase 1 (Atp7a), partial

中文名稱：

Recombinant Rat Copper-transporting ATPase 1(Atp7a) ,partial
貨號：

CSB-YP002414RA
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rat Copper-transporting ATPase 1(Atp7a) ,partial
貨號：

CSB-EP002414RA
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rat Copper-transporting ATPase 1(Atp7a) ,partial
貨號：

CSB-EP002414RA-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rat Copper-transporting ATPase 1(Atp7a) ,partial
貨號：

CSB-BP002414RA
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rat Copper-transporting ATPase 1(Atp7a) ,partial
貨號：

CSB-MP002414RA
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Atp7a
Uniprot No.：

P70705
別名：

Atp7a; MnkCopper-transporting ATPase 1; EC 7.2.2.8; Copper pump 1; Menkes disease-associated protein homolog
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

ATP-driven copper (Cu(+)) ion pump that plays an important role in intracellular copper ion homeostasis. Within a catalytic cycle, acquires Cu(+) ion from donor protein on the cytoplasmic side of the membrane and delivers it to acceptor protein on the lumenal side. The transfer of Cu(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Under physiological conditions, at low cytosolic copper concentration, it is localized at the trans-Golgi network (TGN) where it transfers Cu(+) ions to cuproenzymes of the secretory pathway. Upon elevated cytosolic copper concentrations, it relocalizes to the plasma membrane where it is responsible for the export of excess Cu(+) ions. May play a dual role in neuron function and survival by regulating cooper efflux and neuronal transmission at the synapse as well as by supplying Cu(+) ions to enzymes such as PAM, TYR and SOD3. In the melanosomes of pigmented cells, provides copper cofactor to TYR to form an active TYR holoenzyme for melanin biosynthesis.
基因功能參考文獻：
1. CTR1 and ATP7A play important roles in Cu transport in choroidal epithelial cells, and the Pb-induced intracellular Cu accumulation appears to be mediated, at least in part, via the alteration of CTR1 and ATP7A expression levels following Pb exposure. PMID: 24316150
2. Atp7a knockdown in IEC-6 cells enhanced transepithelial iron flux via a novel molecular mechanism involving transcriptional induction of Fpn1 gene expression. PMID: 24174620
3. Iron chelation with copper loading increased Atp7a mRNA and protein levels. PMID: 23174565
4. Multiple Atp7a transcript and protein variants exist in rodent intestinal epithelial cells and are induced by dietary iron deprivation. PMID: 22579041
5. these results suggest that Ctr1, DMT1, ATOX1 and ATP7A contribute to Cu transport at the blood-CSF barrier PMID: 22442359
6. Data show that the Atp7a gene is upregulated by direct interaction with HIF2alpha, demonstrating coordinate regulation with genes related to intestinal iron homeostasis. PMID: 21346155
7. Conclude that novel Atp7a protein variants may exist in rat intestinal epithelial cells, with different intracellular locations and potentially distinct physiological functions. PMID: 19679821
8. Atp7a plays an important role in copper-dependent vascular smooth muscle cell migration and may contribute to neointimal formation after experimental vascular injury. PMID: 20671235
9. ATP7A is involved in copper loading observed during iron deficiency and increased intestinal copper transport is of physiological relevance PMID: 16081413
10. Immunolocalization of MNK was demonstrated mainly over the trans Golgi network of parotid acinar cells; immature and mature secretory granules were also labelled. PMID: 16185750
11. Prolactin stimulated Cu transport as a result of increased Ctr1 and Atp7A abundance at the plasma membrane in mammary gland PMID: 16741141
12. Intestinal cells contain dispersed vesicular compartment that actively sequesters excess cytoplasmic copper. PMID: 17158254
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亞細胞定位：

Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Melanosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse, postsynaptic density.
蛋白家族：

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IB subfamily
組織特異性：

Expressed in hippocampal neuron (at protein level). Expressed in anterior pituitary gland (at protein level).
數(shù)據(jù)庫鏈接：

KEGG: rno:24941

STRING: 10116.ENSRNOP00000063702

UniGene: Rn.10554