Recombinant Human Secreted and transmembrane protein 1 (SECTM1), partial (Active)

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中文名稱：

人SECTM1重組蛋白
品名簡稱：

Recombinant Human SECTM1 protein, partial (Active)
貨號：

CSB-MP819898HU
說明書：

MSDS Datasheet
規格：

￥588
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized CD7 (CSB-MP004953HU) at 5 μg/ml can bind human SECTM1, the EC₅₀ is 1.811-3.372 ng/ml. Biological Activity Assay

Activity
Human CD7 protein hFc and Myc tag (CSB-MP004953HU) captured on COOH chip can bind Human SECTM1 protein hFc tag (CSB-MP819898HU) with an affinity constant of 1.84 nM as detected by LSPR Assay. Biological Activity Assay
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產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

①Measured by its binding ability in a functional ELISA. Immobilized CD7 (CSB-MP004953HU) at 5 μg/ml can bind human SECTM1, the EC₅₀ is 1.811-3.372 ng/ml.②Human CD7 protein hFc and Myc tag (CSB-MP004953HU) captured on COOH chip can bind Human SECTM1 protein hFc tag (CSB-MP819898HU) with an affinity constant of 1.84 nM as detected by LSPR Assay.
基因名：

SECTM1
Uniprot No.：

Q8WVN6
別名：

(Protein K-12)(K12)
分子結構：
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

41.6 kDa
表達區域：

29-145aa
氨基酸序列

QNEGWDSPICTEGVVSVSWGENTVMSCNISNAFSHVNIKLRAHGQESAIFNEVAPGYFSRDGWQLQVQGGVAQLVIKGARDSHAGLYMWHLVGHQRNNRQVTLEVSGAEPQSAPDTG
蛋白標簽：

C-terminal hFc1-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

CUSABIO cloned the coding sequence of human secreted and transmembrane protein 1 (SECTM1) (amino acids 29-145) to generate a fusion recombinant protein with the Fc portion of human IgG1 tag in mammalian cells. This recombinant SECTM1 protein is an active protein, whose bioactivity has been validated through a functional ELISA (bind to the CD7 with the EC₅₀ of 1.811-3.372 ng/ml) and an LSPR assay (human CD7 captured on COOH chip can bind to this SECTM1 protein with an affinity constant of 1.84 nM). Its purity reaches up to 85%, measured by SDS-PAGE. Due to the glycosylation, this SECTM1 protein migrated to the molecular weight band of about 45 kDa. It contains less endotoxin, <1.0 EU/ug determined by the LAL method. It is in stock now.

SECTM1, the ligand for CD7, plays a role in the co-stimulation and proliferation of T and NK cells. It is up-regulated in many tumors, including breast cancer, prostate cancer, and some myeloid leukemias.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

May be involved in thymocyte signaling.
基因功能參考文獻：
1. CD7 is present on monocytes and tumor macrophages and its ligand, SECTM1, is frequently expressed in corresponding melanoma tissues PMID: 24157461
2. SECTM1 secreted from bone marrow stromal cells may interact with CD7 to influence GM-CSF expression in leukemic cells. PMID: 24211252
3. level of SECTM1 expression is likely to be a key factor in innate immune responses and in the immune tolerance of cancerous cells PMID: 21749909
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Secreted.
蛋白家族：

SECTM family
組織特異性：

Detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. Found in leukocytes of the myeloid lineage, with the strongest expression observed in granulocytes and no detectable expression in lymphocytes. Expressed in thymic
數據庫鏈接：

HGNC: 10707

OMIM: 602602

KEGG: hsa:6398

STRING: 9606.ENSP00000269389

UniGene: Hs.558009