Recombinant Rat Apelin (Apln), partial

中文名稱：

Recombinant Rat Apelin (Apln), partial
品名簡稱：

Recombinant Rat Apln protein, partial
貨號：

CSB-YP001909RA1
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Apln
Uniprot No.：

Q9R0R3
別名：

APJ endogenous ligand
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
來源：

Yeast
分子量：

2.4 kDa
表達區域：

65-77aa
氨基酸序列

QRPRLSHKGPMPF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Endogenous ligand for the apelin receptor (APLNR). Drives internalization of the apelin receptor. Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR. Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis. Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. Plays a role in early coronary blood vessels formation. Mediates myocardial contractility in an ERK1/2-dependent manner. May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior.
基因功能參考文獻：
1. Appropriate concentration of apelin may reduce retinal microglial cells in a rat model of oxygen-induced retinopathy of prematurity. PMID: 29091306
2. Study shows that apelin-13 significantly attenuates cognitive impairments in 6-OHDA-treated animals. The data support the pro-cognitive property of apelin-13 in 6-OHDA-induced cognitive deficit and provided a new pharmacological aspect of the neuropeptide apelin. PMID: 29329678
3. apelin effects on dynamic mechanical characteristics of single ventricular cardiomyocytes PMID: 28935153
4. The present study describes the role of spinal apelin in the processing of neuropathic pain induced by chronic constriction of the sciatic nerve, and elucidates receptor and intracellular mechanisms underlying apelin-mediated pain modulation. PMID: 28627589
5. Apelin is an important regulator of aortic remodeling and adventitial fibrosis and a promising target for vasoprotective therapies. PMID: 27773659
6. Apelin in 40 and 60mug/kg doses increased cardiac contractility, and reduced mean arterial pressure. PMID: 28987634
7. In type 2 diabetic rats, exercise improved body composition, skeletal muscle capillarization and increased plasma apelin levels. PMID: 28164323
8. High fat diet causes significant increases in the expression of apelin and apelin receptor mRNA in the left ventricle. PMID: 27378063
9. Electroacupuncture stimulation could alleviate inflammatory pain, at least in part, by restoring apelin and APJ mRNA and protein expression levels, which are downregulated in the complete Freund's adjuvant pain model. PMID: 27632349
10. Apelin-36-[L28C(30kDa-PEG)] provides a starting point for the development of diabetes therapeutics that are devoid of the blood pressure effects associated with canonical APJ activation PMID: 27994053
11. Apelin stimulates basal plasma cholecystokinin release and some pancreatic and gastric enzymes activity in adult rats. PMID: 27512001
12. The present study suggests a theoretical basis for the effects of apelin13 and provides insight into the potential clinical application of apelin-13. PMID: 27109054
13. It seems that apelin alone may improve cardiac function in hypothyroid rats via genomic pathways. PMID: 27663841
14. apelin-13 promotes H9c2 rat cardiomyocyte hypertrophy via PI3K-Akt-ERK1/2-p70S6K and PI3K-induced autophagy PMID: 26607438
15. PI3K/Akt/FoxO3a signaling may be involved in apelin induced vascular smooth muscle cell migration. PMID: 26494002
16. Apelin controls fetal and neonatal glucose homeostasis and is altered by fetal growth restriction induced by maternal undernutrition. PMID: 26631739
17. Central endogenous apelin mediates stress-induced changes in gastric and colonic motor functions through APJ receptor PMID: 26680735
18. Apelin-13 did increase ERK phosphorylation through the PI3K/Akt and PKC signaling pathways, resulting in changes in Egr-1 expression. PMID: 26546817
19. Reduction of serum apelin plays a role in the pathophysiology and cardiac failure of Goldblatt hypertension. PMID: 25708823
20. Apela binds directly to APJ and acts through the Gi pathway. Apela is expressed exclusively in adult kidney and regulates fluid homeostasis PMID: 25995451
21. Apelin enhances not only the differentiation and metabolic activity of brown adipocytes but also the browning of white adipocytes. PMID: 25931124
22. A high fat diet and chronic stress resulted in significant suppression of the central pressor action of apelin. PMID: 25311903
23. Apelin-13 and APJ in the paraventricular nucleus contribute to hypertension via sympathetic activation and arginine vasopressin release in the paraventricular nucleus PMID: 24995933
24. study indicates that the beta-arrestin-dependent ERK1/2 activation and not the Gi-dependent signaling may participate in K17F-induced BP decrease PMID: 25012663
25. Apelin exerts its effect via its G-protein-coupled receptor (APLNR) and is solely expressed by pulmonary vascular endothelial cells. PMID: 24363088
26. It has been implicated in obesity-related hypertension. PMID: 24770651
27. Apelin-13 inhibits BK(Ca) channel via a PI3-kinase-dependent signaling pathway in cerebral artery VSM cells, which may contribute to its regulatory action in the control of vascular tone. PMID: 24386141
28. The early effect of biliopancreatic diversion on resuming insulin resistance in rats may be associated with rapid modification of neurohormonal mechanisms involving PYY, apelin and visfatin, rather than with caloric restriction as proposed recently. PMID: 24304571
29. Apelin protects sarcoplasmic reticulum function and cardiac performance during I/R by attenuating oxidation of sarco(endo)plasmic reticulum Ca(2+)-ATPase and RyR. PMID: 23771946
30. The mRNA level of apelin and apelin receptor were determined in the heart and aorta tissue by real-time polymerase chain reaction, respectively. It was found that apelin reduces blood pressure in DOCA-salt rats. PMID: 23387534
31. results strongly suggest that apelin is associated with the development of diabetic retinopathy and contributes to changes in the retinas of diabetic rats PMID: 23874984
32. Apelin acts in the subfornical organ to influence neuronal excitability and cardiovascular function. PMID: 23629509
33. Apelin-13 induced the proliferation and migration of vascular smooth muscle cells as well as the upregulation of osteopontin via the upregulation of Egr-1. PMID: 23973488
34. Data show that the expression of apelin mRNA and protein in ischemic and non-ischemic kidneys was increased significantly; the expression of APJ mRNA and protein in ischemic kidneys had no significance, while that in non-ischemic kidneys was decreased. PMID: 23668014
35. Physical exercise decreased apelin expression and elevated APJ expression in pulmonary tissues of rats with hypoxic pulmonary hypertension. PMID: 22493882
36. The glomerular expression of apelin increased significantly in adriamycin (ADR)-induced nephrotic rats and was correlated with urine protein. PMID: 22081427
37. apelin-12 stimulates gastric acid secretion through an increase in histamine release and synthesis from gastric mucosa, suggesting that apelin might play a role in the secretion of gastric acid or serve as a regulating factor of the secretion of gastric acid PMID: 22209991
38. At term, plasma levels of apelin were not modulated by maternal hypertension but APJ expression was increased in placenta and lung but reduced in heart. PMID: 22446510
39. Under hypoxic stress, apelin mRNA expression increased and APJ mRNA also up-regulated, whereas they both down-regulated during glucose deprivation. PMID: 22240274
40. Apelin was also expressed in the neurons of experimental and control rats; it gradually increased in the experimental rat post-seizure and reached a stable high level in chronic epileptic phase. PMID: 21864607
41. these findings highlight the inhibitory role of apelin as an autocrine/paracrine peptide acting on oxytocin neurons during breastfeeding. PMID: 21733827
42. Maternal circulating apelin drops considerably between mid- and late- pregnancy owing to faster clearance. PMID: 20580085
43. While apelin/APJ myocardial expression decreases, apelin plasma levels increase in left ventricular hypertrophy PMID: 20055692
44. Apelin protects against ischemic cardiomyocyte apoptosis via activation of the PI3K/Akt pathway. PMID: 20079140
45. data support a role for the apelin-APJ system in the regulation of smooth muscle, epithelial and goblet cell function in the GI tract PMID: 19660504
46. data suggest a role for apelin in the regulation of vasopressin-containing neurons PMID: 12787050
47. Apelin was shown to stimulate gastric cell proliferation and also stimulated CCK secretion from a murine enteroendocrine cell line (STC-1); apelin-stimulated CCK secretion is mediated through MAPK but not by intracellular Ca(2+) signaling. PMID: 14670994
48. dehydration-induced release of AVP from magnocellular hypothalamic neurons may be responsible for the observed increase in immunoreactive apelin levels within the same neurons PMID: 15166125
49. apelin acts as a potent diuretic neuropeptide counteracting AVP actions through inhibition of AVP neuron activity and AVP release. PMID: 15231996
50. Injections of apelin-13 and apelin-12 (15 microg/kg) into spontaneously hypertensive rats lowered systolic and diastolic blood pressure. Apelin-13(F13A) revealed a loss of function. PMID: 15486224
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亞細胞定位：

Secreted. Secreted, extracellular space.
蛋白家族：

Apelin family
組織特異性：

Expressed in the lung, testis, ovary, uterus and mammary gland. Expressed in neurons in the thalamic paraventricular and hypothalamic supraoptic nuclei. The lung, testis and uterus mainly contain a large form that looks like apelin-36, whereas the mammary
數據庫鏈接：

KEGG: rno:58812

STRING: 10116.ENSRNOP00000005331

UniGene: Rn.22847