Recombinant Mouse Rho-related GTP-binding protein RhoH (Rhoh)

中文名稱：

小鼠Rhoh重組蛋白
貨號：

CSB-YP861532MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Rhoh重組蛋白
貨號：

CSB-EP861532MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Rhoh重組蛋白
貨號：

CSB-EP861532MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Rhoh重組蛋白
貨號：

CSB-BP861532MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Rhoh重組蛋白
貨號：

CSB-MP861532MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Rhoh
Uniprot No.：

Q9D3G9
別名：

Rhoh; Arhh; Rho-related GTP-binding protein RhoH
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-188
氨基酸序列

MLSSIKCVLV GDSAVGKTSL LVRFTSETFP EAYKPTVYEN TGVDVFMDGI QISLGLWDTA GNDAFRSIRP LSYQQADVVL MCYSVANHNS FLNLKNKWIS EIRSNLPCTP VLVVATQTDQ REVGPHRASC INAIEGKRLA QDVRAKGYLE CSALSNRGVQ QVFECAVRTA VNQARRRNRR KLFSINEC
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells.
基因功能參考文獻：
1. These results highlight a novel negative regulatory role for RhoH in eosinophil differentiation, most likely in consequence of altered GATA-2 levels. PMID: 27740624
2. An excess amount of RhoH is able to initiate pre-TCR signaling in the absence of pre-TCR complexes. PMID: 26114424
3. The C-terminal insert domain regulates RhoH protein stability via chaperone-mediated autophagy. PMID: 24189071
4. RhoH plays a critical role in prosurvival chronic lymphocytic leukemia cell-cell and cell-microenvironment interactions with this agent. PMID: 22474251
5. Results suggest that RhoH regulates TCR signaling via recruitment of ZAP-70 and Lck to CD3zeta in the immunological synapse. PMID: 21103055
6. These findings stress the importance of Rhoh for the function of peripheral T cells. PMID: 21182079
7. RhoH has a function in the progression of B-cell chronic lymphocytic leukemia (CLL) in a murine model and show RhoH expression is altered in human primary lymphocytic (CLL) samples. PMID: 19847197
8. RhoH serves as a negative regulator of both growth and actin-based function of hematopoietic progenitor cells possibly via suppression of Rac-mediated signaling. PMID: 15494435
9. RhoH is a critical regulator of thymocyte development and TCR signaling by mediating recruitment and activation of Zap70. PMID: 17028588
10. RhoH is important for pre-T-cell receptor and T-cell receptor signaling PMID: 17119112
11. An antagonistic role of RhoH in regulation of cortical F-actin assembly and chemotaxis via suppressing Rac1 membrane targeting and activation in primary hematopoietic progenitor cells, is identified. PMID: 18089848
12. RhoH plays critical roles in mast cell function by facilitating phosphorylation of Syk kinase in Fc epsilon I receptor-dependent signal transduction. PMID: 19124738
13. RhoH/TTF represents an inducible feedback inhibitor in neutrophils that is involved in the limitation of innate immune responses. PMID: 19414807
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亞細胞定位：

Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Colocalizes together with ZAP70 in the immunological synapse.
蛋白家族：

Small GTPase superfamily, Rho family
組織特異性：

Expression is widespread in hematopoietic cells, including in bone marrow progenitor cells and in differentiated myeloid as well as lymphoid cells. Expressed at high levels in the thymus and mast cells, found in spleen and low-density bone marrow (LDBM) c
數據庫鏈接：

KEGG: mmu:74734

STRING: 10090.ENSMUSP00000031106

UniGene: Mm.20323