Recombinant Mouse Neuronal PAS domain-containing protein 2 (Npas2), partial

中文名稱：

小鼠Npas2重組蛋白
貨號：

CSB-YP015967MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Npas2重組蛋白
貨號：

CSB-EP015967MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Npas2重組蛋白
貨號：

CSB-EP015967MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Npas2重組蛋白
貨號：

CSB-BP015967MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Npas2重組蛋白
貨號：

CSB-MP015967MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Npas2
Uniprot No.：

P97460
別名：

Npas2; Neuronal PAS domain-containing protein 2; Neuronal PAS2
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina. NPAS2 plays an important role in sleep homeostasis and in maintaining circadian behaviors in normal light/dark and feeding conditions and in the effective synchronization of feeding behavior with scheduled food availability. Regulates the gene transcription of key metabolic pathways in the liver and is involved in DNA damage response by regulating several cell cycle and DNA repair genes. Controls the circadian rhythm of NR0B2 expression by binding rhythmically to its promoter. Mediates the diurnal variation in the expression of GABARA1 receptor in the brain and contributes to the regulation of anxiety-like behaviors and GABAergic neurotransmission in the ventral striatum.
基因功能參考文獻：
1. Here we have provided an initial key insight into the interplay between neonatal establishment of the peripheral circadian clock in the liver and the ability of the gut microbiome to respond to dietary and metabolic stress. PMID: 28373017
2. In CLOCK-deficient fibroblasts, knockdown of Npas2 leads to arrhythmicity, suggesting that NPAS2 can compensate for loss of CLOCK in peripheral cells as well as in suprachiasmatic nucleus. PMID: 26895328
3. Study identified a unique role for Npas2 in the regulation of cocaine reward and dopamine Drd3 receptor expression PMID: 25444159
4. In the absence of NADPH, a change in pH from 6.5 to 8.0 decreased the KD(app) value of the E-box from 125 to 22 nM, with an 8-fold increase in the maximal level of DNA binding for the NPAS2/BMAL1 heterodimer. PMID: 25526362
5. Shp and Npas2 crosstalk is essential to maintain hepatic lipid homeostasis. PMID: 25212631
6. Dysregulation of Npas2 leads to altered metabolic pathways in a murine knockout model. PMID: 24067359
7. BMAL1 in a complex with CLOCK or NPAS2 regulates cerebral redox homeostasis and connects impaired clock gene function to neurodegeneration. PMID: 24270424
8. Npas2 shows circadian expression in retinal ganglion cells in mice.And Contrast sensitivity is similarly reduced in Npas2-/- mice. PMID: 24048828
9. NPAS2 containing the N-terminal 61 residues forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like protein (BMAL1) to bind DNA; NAD(P)H enhances the binding activity. PMID: 23831463
10. DNA-binding of NPAS2 and BMAL1 to Per2 was also decreased by SD, although SD is known to increase Per2 expression in the cortex. DNA-binding to Per1 and Cry1 was not affected by SD PMID: 22039518
11. NPAS2 deletion produced significant impairment in the ability of the knockout mice to maintain body weight during restricted feeding and overnight food deprivation. PMID: 20026146
12. results indicate that NPAS2 plays a substantive role in maintaining circadian behaviors in normal light:dark cycle and feeding conditions and that NPAS2 is critical for adaptability to food restriction PMID: 12843397
13. CO binding disrupts the hydrogen bonding of His171 of NPAS2 with surrounding amino acids, which induces conformational changes in the His171-Cys170 moiety, leading to physiological signaling PMID: 15797872
14. overexpressed wild-type and His mutants of the PAS-B domain (residues 241-416) of mouse NPAS2 and then purified and characterized the isolated heme-bound proteins PMID: 16098197
15. the CLOCK(NPAS2)/BMAL1 complex is post-translationally regulated by cry1 and cry2 PMID: 16628007
16. Results suggest that NPAS2 plays a role in sleep homeostasis, most likely at the level of the thalamus and cortex, where NPAS2 is abundantly expressed. PMID: 16636276
17. Brain protein domain binds specifically to the E-box DNA sequence in the presence, but not in the absence, of heme. PMID: 16704425
18. NPAS2 (MOP4) is able to functionally in the master brain clock in mice to regulate circadian rhythmicity. PMID: 17417633
19. Npas2 maintains rhythmic suprachiasmatic nucleus function in the absence of Clock, but Npas2 alone is unable to maintain rhythmicity in peripheral tissues. PMID: 17637349
20. These results indicate that the transcriptional activities of the mutants correlated well with their DNA-binding activities. PMID: 18230344
21. There is an overlapping role of CLOCK and NPAS2 in the regulation of circadianly controlled genes. PMID: 18316400
22. Mutation of CLOCK abolished the diurnal variation in thrombotic occlusion, whereas deletion of NPAS2 altered its temporal pattern; NPAS2 deletion prolonged it and reduced blood pressure irrespective of clock time PMID: 18413500
23. Neuronal PAS protein 2-Per2 protein is a heme-sensor protein in which teine-215 is the heme ligand. PMID: 18479150
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亞細胞定位：

Nucleus.
組織特異性：

Expressed in the retinal ganglion cells (at protein level). Expressed in the hypothalamic suprachiasmatic nuclei (SCN) of the brain. Also found in spinal cord, and to a lesser extent in colon, small intestine and uterus. Exhibits a diurnal variation in it
數據庫鏈接：

KEGG: mmu:18143

STRING: 10090.ENSMUSP00000054719

UniGene: Mm.2380