Recombinant Mouse Interleukin-12 subunit beta (Il12b)

中文名稱：

小鼠Il12b重組蛋白
貨號(hào)：

CSB-YP011587MO
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Il12b重組蛋白
貨號(hào)：

CSB-EP011587MO
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Il12b重組蛋白
貨號(hào)：

CSB-EP011587MO-B
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Il12b重組蛋白
貨號(hào)：

CSB-BP011587MO
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Il12b重組蛋白
貨號(hào)：

CSB-MP011587MO
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Il12b
Uniprot No.：

P43432
別名：

Il12bInterleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length of Mature Protein
表達(dá)區(qū)域：

23-335
氨基酸序列

MWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
基因功能參考文獻(xiàn)：
1. In macrophages, SCIMP is constitutively associated with the Lyn tyrosine kinase, is required for tyrosine phosphorylation of TLR4, and facilitates TLR-inducible production of the IL-6 and IL-12p40. PMID: 28098138
2. Results show the overproduction of IL-12/23p40 in dendritic cells, leading to Th1 and Th17 programs in a mouse model of Yersinia enterocolitica-induced reactive arthritis. PMID: 29494692
3. this study demonstrates that regulation of IL-12p40 by hypoxia-inducible factor-1alpha controls Th1/Th17 responses to prevent mucosal inflammation PMID: 28120851
4. the nuclear localized CA-VI B selectively promotes IL-12 expression by interaction with protein arginine N-methyltransferase 5 (PRMT5), which reduces symmetric dimethylation of histone H3 arginine 8 modification (H3R8me2s) at Il12 promoters to facilitate chromatin accessibility, selectively enhancing c-Rel binding to the Il12b promoter. PMID: 28739930
5. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
6. IL-12p40 depletion in mice could promote ectopic bone formation. PMID: 27472064
7. Data indicate that there was a differential transcription of 381 genes which changed greater than two fold in B1a of p40-/-IL-2Ralpha-/- mice. PMID: 27105495
8. this study shows that endosomal signalling by Lactobacillus acidophilus that leads to IL-12 production is inhibited by TLR stimulation from the plasma membrane PMID: 27441725
9. Furthermore, IL-12p40-/- mice showed decreased sensitization to inhaled cockroach antigen after PVM infection when compared to wild-type mice. PMID: 27400340
10. The study demonstrate a protective role of IL-12Rbeta2 in relapsing-remitting experimental autoimmune encephalomyelitis. PMID: 26857496
11. Interleukin-12/23p40 plays an essential role in the development of graft-versus-host disease in mice. PMID: 25846718
12. Data indicate that sera from treated with cholesterol-p40 (Il12b)-siRNA contained significantly reduced concentrations of p40. PMID: 26232431
13. this study demonstrates viral RNA analog-induced transcription synergy of Il6 and Il12b via IFN regulatory factor (IRF)1 (TLR3-TIR domain-containing adaptor inducing IFN-beta [TRIF] responsive), C/EBPbeta (TLR7-MyD88 responsive), and JunB (all responsive). PMID: 26109639
14. our results suggest that IL-12p40 acts as a negative regulator of autoimmune cholangitis in IL-2Ralpha-/- mice. Compared to IL-2Ralpha-/- mice, p40-/-IL-2Ralpha-/- mice develop liver fibrosis and reduced colitis. PMID: 24651036
15. These results imply that a soluble host or Toxoplasma gondii factor is responsible for the bulk of IL-12p40 production in vivo. PMID: 25024368
16. the assembled p40-CD5L heterodimer does not recapitulate the biological activity of IL-12. PMID: 24821971
17. The immune-enhancing effect of the Cronobacter sakazakii ES2 phage results in the activation of nuclear factor-kappaB and dendritic cell maturation via the activation of IL-12p40 in the mouse bone marrow. PMID: 24184907
18. IL-12 does not induce the expression of IL-7 in microglia and macrophages via both IL-12Rbeta2 and IL-12Rbeta1. PMID: 24224652
19. Data indicate that the GCN2 knockout mice showed reduced inflammatory responses, with decreased IL-6 and IL-12 expression correlating with significant reduction in animal mortality. PMID: 24248597
20. IL-12p40 contributed most in inducing Th1 and Th17 polarization PMID: 23697689
21. IL-12p40 deficiency leads to uncontrolled Trypanosoma cruzi dissemination in the spinal cord resulting in neuronal death and motor dysfunction. PMID: 23152844
22. Klf10 is a transcription factor that regulates the expression of IL-12p40 in bone marrow-derived macrophages. PMID: 23065757
23. IL-18 and IL-12 exert a synergistic effect on the lungs by inducing MMPs, cathepsins S, and pro-inflammatory cytokines, which may promote pulmonary emphysema and inflammation. PMID: 22908875
24. Inhibition of IL-12/IL-23 signaling reduces Alzheimer's disease-like pathology and cognitive decline. PMID: 23178247
25. Novel IL27p28/IL12p40 cytokine suppressed experimental autoimmune uveitis by inhibiting autoreactive Th1/Th17 cells and promoting expansion of regulatory T cells. PMID: 22936807
26. TLR2 plays a dual role in regulating TLR9-dependent IL-12p40 production in dendritic cells. PMID: 22635254
27. in mice deficient in IL-12p40, human metapneumovirus infection induced an exacerbated pulmonary inflammatory response and mucus production, altered cytokine response, and decreased lung function PMID: 22606349
28. reactive oxygen species are important for IL-12 production induced by lactobacillus PMID: 22536449
29. MKP-1 increases the expression of IL-12 by enhancing the expression of IRF1 PMID: 22464096
30. extracellular signal regulated kinases modulate IL-12 expression by regulating IkappaB-zeta-dependent binding of NF-kappaB transcription factors to IL-12b gene promoter PMID: 22131231
31. Francisella tularensis LVS induced innate production of IL-12p(40)2 is required for dendritic cell migration. PMID: 21669537
32. microbiota negatively regulates host miR-10a expression, which may contribute to the maintenance of intestinal homeostasis by targeting IL-12/IL-23p40 expression. PMID: 22068236
33. Data show that the p40 vaccine induced specific antibodies to IL-12 and IL-23, which was associated with improvement of intestinal inflammation and fibrosis. PMID: 21424108
34. dendrittic cells-derived, controls acute infection by Toxoplasma gondii PMID: 21867928
35. MW and glucocorticoids independently modulate IL-12p40 production through a mechanism involving ERK1/2 hyperactivation and that glucocorticoids can significantly augment MW-induced inhibition of IL-12p40. PMID: 21730055
36. A distal enhancer in Il12b is the target of transcriptional repression by the STAT3 pathway and requires the basic leucine zipper (B-ZIP) protein NFIL3. PMID: 21566115
37. Data identified NFIL3 as a regulatory transcription factor in macrophages, controlling IL-12 p40 production. PMID: 21383239
38. The colitis-prone SJL/J-derived polymorphic variant of IL12b p40 more readily forms p70 heterodimers than does the colitis-resistant C57BL/6 strain-derived p40 variant. PMID: 21321105
39. PKCdelta is a regulator of IL-12p40/p70 production by dendritic cells and macrophages, driving the healer phenotype during cutaneous leishmaniasis. PMID: 21287553
40. macrophage-derived Arg I protects hosts against excessive tissue injury caused by worm eggs during acute schistosomiasis by suppressing IL-12/IL-23p40 PMID: 20483789
41. CD14(neg) B6 mice showed delayed production of IL12p40 in response to necrotic cells PMID: 19840871
42. These studies demonstrated that IL-12 plays a predominant role in the expansion of effector CD8(+) T-cell immunity against Encephalitozoon cuniculi, which is critical for host survival. PMID: 20308292
43. there is a critical role for the tyrosine phosphatase activity of SHP-1 for induction of IL-12p40 production in macrophages in response to TLR ligands PMID: 20145200
44. Results show that IL-12-->IL-27 sequential gene therapy provides a simple but effective approach for eliminating inaccessible residual tumors. PMID: 20139275
45. IL-12p40 induction in macrophages is directly regulated by the intracellular glutathione-redox status of mouse macrophages in vitro. PMID: 20164428
46. We suggest that overproduction of NO may account for neuronal death at the spinal cord of T. cruzi-infected IL-12p40KO mice and that IFN-gamma and S100beta may contribute to NOS activation in the absence of IL-12. PMID: 19923851
47. We found that the production of IL-12 preceded that of other cytokines in response to Influenza A virus lung infection. PMID: 19941386
48. Langerin-positive cells are not required for initial activation of invariant natural killer T (iNKT) cells, but are required for downstream release of large quantities of IL-12p70 into the serum. PMID: 19923446
49. protective and agonistic function in mycobacterial infection PMID: 11739515
50. Heat shock inhibits IL-12 p40 expression through NF-kappa B signalling pathway in murine macrophages PMID: 11792125
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Type I cytokine receptor family, Type 3 subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:16160

STRING: 10090.ENSMUSP00000099860

UniGene: Mm.239707