Recombinant Mouse Exonuclease 1 (Exo1), partial

中文名稱：

小鼠Exo1重組蛋白
貨號：

CSB-YP007876MO
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Exo1重組蛋白
貨號：

CSB-EP007876MO
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Exo1重組蛋白
貨號：

CSB-EP007876MO-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Exo1重組蛋白
貨號：

CSB-BP007876MO
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Exo1重組蛋白
貨號：

CSB-MP007876MO
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Exo1
Uniprot No.：

Q9QZ11
別名：

Exo1; Exonuclease 1; mExo1; EC 3.1.-.-; Exonuclease I
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
基因功能參考文獻：
1. like canonical DNA mismatch-repair proteins, EXO1 can restrain aberrant homologous recombination events between diverged sequence elements in the genome. PMID: 28711786
2. The results establish a key role for EXO1 in modulating the severity of hypomorphic MRE11 complex mutations. PMID: 26160886
3. Exo1-mediated HR is dispensable for stem cell function in quiescent HSC, whereas it is essential to HSC response to DNA damage processing after cell cycle entry, and its loss is not compensated by intact NHEJ PMID: 24420907
4. In contrast to Exo1(null/null) mice, Exo1(E109K/E109K) knockin mice retain mismatch repair activity and display normal class switch recombination and meiosis. PMID: 23754438
5. RNAi-mediated EXO1 knockdown in mouse fibroblasts directly results in an alkylation-tolerant phenotype. PMID: 23062884
6. Exo1 contributes to the metabolism of DNA ends during DNA double-strand breaks repair in B lymphocytes. PMID: 23254285
7. Study documents the combinatorial action of Apollo, POT1b, CST, and the 5' exonuclease Exo1 in postreplicative telomere end processing in mouse cells, clarifying the mechanism by which the telomeric 3' overhang is generated and modulated. PMID: 22748632
8. EXO1 can convert DNA nicks and point mutations into double-stranded DNA breaks for both core nonhomologous end-joining factors and alternative end-joining pathways of class-switch recombination. PMID: 21242524
9. Functions in mutation avoidance and is essential for male and female meiosis. PMID: 12629043
10. Apc(1638N) Exo1 Fen1 mice survive longer (18 months) PMID: 17452984
11. EXO1 contributes to DNA damage signal induction in mammalian cells, and deletion of Exo1 can prolong survival in the context of telomere dysfunction. PMID: 17803909
12. Meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway. PMID: 18057311
13. Switch Junction Position Is Altered in Exo1-/- Mi PMID: 18940926
14. ablation of either Msh6 or Exo1 function in the Mgmt-null mouse renders these rapidly proliferating tissues alkylation-resistant. The Exo1 defect leads to a variable tissue-specific alkylation resistance phenotype PMID: 19124772
15. Mlh1 or Exo1 deficiencies also eliminate class switch recombination in the absence of the SmuTR PMID: 19553545
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亞細胞定位：

Nucleus.
蛋白家族：

XPG/RAD2 endonuclease family, EXO1 subfamily
組織特異性：

Highly expressed in the spleen and testis. Also expressed in the bone marrow, brain, lung, lymph node and thymus.
數據庫鏈接：

KEGG: mmu:26909

STRING: 10090.ENSMUSP00000039376

UniGene: Mm.283046