Recombinant Human Protein arginine N-methyltransferase 1 (PRMT1), partial

中文名稱：

Recombinant Human Protein arginine N-methyltransferase 1 (PRMT1), partial
品名簡稱：

Recombinant Human PRMT1 protein, partial
貨號：

CSB-EP859113HU1c7
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

PRMT1
Uniprot No.：

Q99873
別名：

Histone-arginine N-methyltransferase PRMT1;Interferon receptor 1-bound protein 4
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

35.0 kDa
表達區(qū)域：

1-248aa
氨基酸序列

MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner. Plays a role in regulating alternative splicing in the heart.
基因功能參考文獻：
1. Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives. PMID: 29540535
2. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. PMID: 29651020
3. Study characterizes a novel splicing isoform of PRMT1 that correlates with cellular malignancy. This isoform lacks the dimerization arm of the enzyme, and is catalytically inactive, but able to bind substrates, and blocks cells in the G1 phase of the cell cycle. PMID: 28857308
4. the abnormal stable complex of FUS-R521C/PRMT1/Nd1-L mRNA could contribute to neurodegeneration upon oxidative stress. PMID: 28094300
5. Important roles of PRMT1 in progression of gastric cancer. Given the dual functions of PRMT1, it is as a potential drug target of gastric cancer with extreme caution. PMID: 29097184
6. Mitochondrial Ca(2+) uptake is controlled by protein arginine methyl transferase 1 that asymmetrically methylates MICU1, resulting in decreased Ca(2+) sensitivity. UCP2/3 normalize Ca(2+) sensitivity of methylated MICU1 and, thus, re-establish mitochondrial Ca(2+) uptake activity. PMID: 27642082
7. High-PRMT1 expression is associated with resistance to cetuximab in triple-negative breast cancer. PMID: 28643125
8. a significant role for PRMT1 in HCC progression and metastasis in vitro and in vivo via STAT3 signalling pathway activation. PRMT1 may be a potential novel prognostic biomarker and new therapeutic target for hepatocellular carcinoma. PMID: 29945155
9. High PRMT1 expression is associated with head and neck and oral cancer. PMID: 28656289
10. TRIM48 Promotes ASK1 Activation and Cell Death through Ubiquitination-Dependent Degradation of the ASK1-Negative Regulator PRMT1 PMID: 29186683
11. data collectively suggest that silencing of PRMT1 exerts anti-catabolic and anti-inflammatory effects on IL-1beta-induced chondrocytes via suppressing the Gli-1 mediated Hedgehog signaling pathway, indicating that PRMT1 plays a critical role in OA development and serves as a promising therapeutic target for OA. PMID: 28744817
12. Deacetylation on both K200 and K205 by Sirtuin 1 (Sirt1) and acetylation of p300 (EP300) on K205 collaboratively prepare the motif for SCF(Fbxl17) binding thereby triggering PRMT1 protein degradation. PMID: 28883095
13. PRMT1 has a role in regulating MYCN in neuroblastoma PMID: 27571165
14. Analysis of deletion constructs of SERBP1 showed that the C-terminal third of the SERBP1 protein, which contains one of its two substrate sites for protein arginine N-methyltransferase 1 (PRMT1), is necessary and sufficient for it to interact with RACK1 PMID: 28267599
15. PRMT1 is an arginine methyltransferase involved in a variety of cell functions. Here the authors delete PRMT1 specifically in mature B cells to show the importance of arginine methylation for B cell proliferation, differentiation and survival, and thereby for humoral immunity. PMID: 29026071
16. High PRMT1 expression is associated with multidrug resistance in breast cancer. PMID: 26934120
17. IFNgamma induced PPAR gamma coactivator-1 alpha (PGC-1alpha) positively regulated RIG-I; with PRMT-1 and G9a affecting PGC-1alpha in a counter-regulatory manner. PMID: 26725954
18. PRMT1 inhibition prevents gastric cancer progression by downregulating eIF4E and targeting type II PRMT5. PMID: 28987382
19. RALY promotes Protein Arginine Methyltransferase 1 alternatively spliced isoform v2 relative expression and metastatic potential in breast cancer cells PMID: 28733251
20. the two structural homologs FAM98A and FAM98B included in a novel complex with DDX1 and C14orf166 are required for PRMT1 expression in colorectal cancer cell lines PMID: 28040436
21. Maintenance of the progenitors thus requires cooperation by PRMT1 and CSNK1a1 to sustain proliferation gene expression and suppress premature differentiation driven by GRHL3 PMID: 28943242
22. PRMT1 methylates Nrf2 protein at a single residue of arginine 437, both in vitro and in vivo. This leads to a moderate increase of its DNA-binding activity and transactivation, which subsequently protects cells against the tBHP-induced glutathione depletion and cell death. PMID: 27183873
23. Our data suggest that miR-19a and PRMT1 might be novel indicators of asthma and present new disease-specific therapeutic targets to reduce airway wall remodeling and inflammation in asthmatic patients PMID: 28081849
24. The PRMT1 active site residues, Met48 and His293, have been determined to play a key role in dictating product specificity, as: (1) the single mutation of Met48 to Phe enabled PRMT1 to generate MMA, ADMA, and a limited amount of SDMA; (2) the single mutation of His293 to Ser formed the expected MMA and ADMA products only; (3) the double mutant H293S-M48F-PRMT1 produced SMDA as the major product with limit amount of SDMA. PMID: 27545444
25. This article reviews the current literature from research showing interdependent association between cav1-PRMT1-SIRT1 to the outcomes of experimental and clinical research aiming to preserve endothelial function with gene- or pharmaco-therapy. [review] PMID: 28126510
26. Low expression of PRMT1 is associated with low chemosensitivity in gastric cancer. PMID: 26472729
27. PRMT1 is overexpressed in human melanoma, and may regulate tumor growth and metastasis via targeting ALCAM. PMID: 27175582
28. Protein arginine N-methyltransferase 1 expression was found to be significantly upregulated in hepatocellular carcinoma cell lines and clinical tissues. PMID: 28231732
29. FAM98A, whose physiological function is unknown, was arginine-methylated by PRMT1. PMID: 26503212
30. PRMT1 promoted the methylation of Gli1. PMID: 26762411
31. The oncogenic roles of PRMT1 in the progression of ESCC. PMID: 26392112
32. we unraveled a dual function of PRMT1 in modulation of both EMT and senescence via regulating ZEB1. PMID: 26813495
33. Protein arginine methyltransferase 1 is regulated by the Interleukin-4/STAT6 signal pathway in epithelial cells, while in fibroblasts PRMT1 is activated by Interleukin-1 beta through NF-kappa B. PMID: 26911452
34. Upon HBV infection, cellular mechanisms involving SETDB1-mediated H3K9me3 and HP1 induce silencing of HBV cccDNA transcription through modulation of chromatin structure. PMID: 26143443
35. This study demonstrated that Loss of PRMT1 not only results in an increase of UMA FUS and a decrease of ADMA FUS, but also in a significant increase of MMA FUS. PMID: 26895297
36. findings suggest that PRMT1 is a central regulator of tissue remodeling and that the signaling sequence controlling its expression in primary human lung fibroblast is PDGF-ERK-STAT1. PMID: 26795953
37. The authors demonstrate that RBM15 is methylated by protein arginine methyltransferase 1 (PRMT1) at residue R578, leading to its degradation via ubiquitylation by an E3 ligase (CNOT4). PMID: 26575292
38. Data unveils a novel mechanism of PRMT1-mediated CPC regulation through methylation of INCENP. PMID: 26460953
39. there is an imbalance in the vasoconstriction-vasodilation status toward constriction. It is our hypothesis that, in hypoxic stress, a key player in initiating this imbalance is the enzyme, protein arginine methyltransferase-1 (PRMT1). PMID: 26527496
40. Pharmacological inhibition of KDM4C/PRMT1 suppresses transcription and transformation ability of MLL fusions PMID: 26766589
41. Methylation by PRMT1 may regulate Smurf2 stability and control TGF-beta signaling. PMID: 26126536
42. PRMT1 regulates glucose-stimulated insulin secretion through enhanced methylation-induced nuclear localization of FOXO1, which subsequently suppresses the nuclear localization of PDX-1 PMID: 25874535
43. we define the arginines within the RGG/RG motifs as the site of methylation by PRMT1 both in vitro and in vivo. The arginines within the human hnRNPUL1 RGG/RG motifs were substituted with lysines to generate hnRNPUL1(RK). PMID: 26020839
44. PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response. PMID: 26571401
45. miR-503-mediated PRMT1 could emerge as a potential important biomarker for hepatocellular carcinoma progression and metastasis PMID: 26163260
46. results challenge the unilateral view that increased PRMT1 expression necessarily results in increased ADMA synthesis and demonstrate that enzymatic activity can be regulated in a redox-sensitive manner PMID: 25911106
47. High PRMT1 expression is associated with Epithelial-Mesenchymal-Transition and metastasis in Non-small Cell Lung Cancer. PMID: 25847239
48. The ATXN2L associates with the protein arginine-N-methyltransferase 1 (PRMT1). PMID: 25748791
49. Study provides evidence for loss of PRMT1 function as a consequence of cytoplasmic accumulation of FUS in the pathogenesis of amyotrophic lateral sclerosis, including changes in the histone code regulating gene transcription. PMID: 25274782
50. GSK3- and PRMT-1-dependent modifications of desmoplakin control desmoplakin-cytoskeleton dynamics. PMID: 25733715
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亞細胞定位：

Nucleus. Nucleus, nucleoplasm. Cytoplasm. Cytoplasm, cytosol.
蛋白家族：

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
組織特異性：

Widely expressed. Expressed strongly in colorectal cancer cells (at protein level). Expressed strongly in colorectal cancer tissues compared to wild-type colon samples (at protein level). Expressed strongly in colorectal cancer tissues compared to wild-ty
數(shù)據(jù)庫鏈接：

HGNC: 5187

OMIM: 602950

STRING: 9606.ENSP00000406162

UniGene: Hs.20521