Recombinant Human Protein AATF (AATF), partial

中文名稱：

Recombinant Human Protein AATF(AATF),partial
品名簡稱：

Recombinant Human AATF protein, partial
貨號：

CSB-RP052644h
說明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

AATF
Uniprot No.：

Q9NY61
別名：

AATF; AATF_HUMAN; Apoptosis antagonizing transcription factor ; Apoptosis-antagonizing transcription factor; BFR2; CHE 1; CHE1; DED; Protein AATF; Rb binding protein Che 1; Rb-binding protein Che-1
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
來源：

E.coli
分子量：

58.8kDa
表達區(qū)域：

6-294aa
氨基酸序列

PLALQLEQLLNPRPSEADPEADPEEATAARVIDRFDEGEDGEGDFLVVGSIRKLASASLLDTDKRYCGKTTSRKAWNEDHWEQTLPGSSDEEISDEEGSGDEDSEGLGLEEYDEDDLGAAEEQECGDHRESKKSRSHSAKTPGFSVQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLTTNQLPQPDVFPLFKDKGGPEFSSALKNSHKALKALLRSLVGLQE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal GST-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
基因功能參考文獻：
1. Results show that Che-1 protects colon cancer cells from apoptosis induced by hypoxia through its ability to regulate HIF1-alpha stabilization in colorectal cancer cells. PMID: 28214471
2. Results show that eEF1Bgamma binds to the Che-1 promoter region and its transcript, and describe a novel mitochondrial localization for the Che-1 protein which needs mitochondrial integrity for correct localization. PMID: 27639846
3. we identified the ANN complex as a novel functional module supporting the nucleolar maturation of 40S ribosomal subunits. Our data help to explain the described role of AATF in cell proliferation during mouse development as well as its requirement for malignant tumor growth. PMID: 27599843
4. the effect of APOBEC3G over-expression upon AATF gene expression, was examined. PMID: 27611213
5. loss of Che-1 inhibits proliferation and promotes apoptosis in MG-63 cells by decreasing the level of mutant p53 PMID: 27012205
6. It was concluded that PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of apoptosis antagonizing transcription factor through PARylation. PMID: 26822515
7. These results confirm Che-1 as an important regulator of p53 activity and suggest Che-1 to be a promising yet attractive drug target for cancer therapy. PMID: 25996291
8. In the face of high glucose threat, mitochondrial UCP2 gene expression is regulated by miR-2909 and AATF. PMID: 25976474
9. This mutant AATF along with its interactome consisting of SP1, DNMT3B and Par-4 ensures cancer cell DNA methylation required for down-regulation of tumor suppressor genes. PMID: 25231211
10. HIPK2 depletion strongly decreases Che-1 ubiquitylation and degradation. PMID: 25210797
11. Che-1 expression correlates with the progression of multiple myeloma and is required for cell growth and survival.Che-1 controls mTOR through the induction of Redd1 and Deptor, two important repressors of mTOR. PMID: 25770584
12. Cell proliferation decreased by 41% which was accompanied by apoptosis induction in 30% MCF-7 cells after AATF gene knockdown. PMID: 23801113
13. Che-1 depletion abolishes the ability of Chk1 to bind pericentrin and to localize at centrosomes, which, in its turn, deregulates the activation of centrosomal cyclin B-Cdk1 and advances entry into mitosis. PMID: 23798705
14. These results identify AATF as a nucleolar-confined c-Jun cofactor whose expression levels and spatial distribution determine the stress-induced activity of c-Jun and the levels of c-Jun-mediated apoptosis. PMID: 22933572
15. Nuclear AATF enrichment is selected for in p53-proficient endometrial cancers. Focal copy number AATF gains correlate with reduced overall survival in neuroblastoma. AATF is a critical repressor of p53-driven apoptosis. PMID: 22909821
16. found that Che-1 is required for sustaining mutant p53 expression in several cancer cell lines, and that Che-1 depletion by siRNA induces apoptosis both in vitro and in vivo PMID: 20708154
17. No evidence for the association of mutations with breast cancer was observed. PMID: 20025740
18. Che-1 affects cell growth by interfering with the recruitment of HDAC1 by retinoblastoma protein. Che-1 overexpression activates DNA synthesis in quiescent NIH-3T3 cells through HFDAC1 displacement. PMID: 12450794
19. Che-1 can be considered a general HDAC1 competitor and its down-regulation is involved in colon carcinoma cell proliferation PMID: 12847090
20. apoptosis-antagonizing transcription factor binds to TSG101 in a process that enhances androgen receptor-mediated transcription by promoting its monoubiquitination PMID: 14761944
21. AATF gene may be of crucial importance in maintaining the leukemic state of a cell compartment through its ability to initiate cell proliferation coupled with repression of cellular apoptosis. PMID: 17006618
22. Che-1 as a new Pin1 and HDM2 target and confirm its important role in the cellular response to DNA damage. PMID: 17468107
23. Che-1 interacts with NRAGE and NRAGE overexpression downregulates endogenous Che-1 by targeting it for proteasome-dependent degradation. PMID: 17488777
24. This review explains the novel miRNA encoded exclusively by HIV-1 genome that has the ability to specifically target cellular AATF gene known to play a crucial role in the maintenance of adaptive immunity at nucleic acid level against HIV-1 invasion. PMID: 18341201
顯示更多

收起更多
亞細胞定位：

Nucleus, nucleolus.
蛋白家族：

AATF family
組織特異性：

Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
數(shù)據(jù)庫鏈接：

HGNC: 19235

OMIM: 608463

KEGG: hsa:26574

STRING: 9606.ENSP00000225402

UniGene: Hs.195740