Recombinant Human DNA topoisomerase 2-beta (TOP2B), partial

貨號：

CSB-YP717547HU
規(guī)格：
來源：

Yeast
其他：

貨號：

CSB-EP717547HU
規(guī)格：
來源：

E.coli
其他：

貨號：

CSB-EP717547HU-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

貨號：

CSB-BP717547HU
規(guī)格：
來源：

Baculovirus
其他：

貨號：

CSB-MP717547HU
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

TOP2B
Uniprot No.：

Q02880
別名：

DNA topoisomerase 2-beta; EC 5.6.2.2; DNA topoisomerase II, beta isozyme; TOP2B
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
表達區(qū)域：

736-1189
氨基酸序列

IPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand.
基因功能參考文獻：
1. our data primarily indicate that topoIIbeta can be used to estimate neuronal differentiation in medulloblastoma PMID: 28554742
2. The article discusses origin of topoisomerase II beta, its structure, activities reported in vitro and in vivo along with implications in cellular processes namely transcription, DNA repair, neuronal development, aging, HIV-infection and cancer. (Review) PMID: 28669856
3. recent data point to a critical role of topoisomerase II beta (TOP2B), which is a primary target of anthracycline poisoning, in the pathophysiology of anthracycline-induced congestive heart failure . PMID: 28102848
4. The decatenation checkpoint is regulated, not only by topo IIalpha, as previously reported, but also by topo IIbeta. The decatenation checkpoint is most efficient when both isoforms are present. Deletion of most of the C-terminus of topo IIbeta, while preserving the nuclear localization signal, and mutation of Y656 in topo IIbeta inhibits the decatenation checkpoint and sensitivity to topo II-targeted drugs. PMID: 28472494
5. TOP2B is positioned to solve topological problems at diverse cis-regulatory elements and its occupancy is a highly ordered and prevalent feature of CTCF/cohesin binding sites that flank TADs. PMID: 27582050
6. TopoIIbeta is involved in the cascade of coactivator complexes that are recruited to LTR for regulation of HIV-1 transcription. PMID: 26876283
7. A direct interaction between Ku70/86 and BRG1 brings together SWI/SNF remodeling capabilities and TOP2beta activity to enhance the transcriptional response to hormone stimulation. PMID: 26055322
8. Human cytomegalovirus IE1 exon 4 interacts with Topoisomerase IIbeta (TOPOIIbeta), whose activity is required for viral genome persistence and maintenance via binding to a cis-acting viral maintenance element. PMID: 25011107
9. This study demonstrated that top IIbeta overexpression is necessary for RA-induced neuronal differentiation. PMID: 24801450
10. Data suggest that DNA topoisomerase IIbeta (topoIIbeta) silencing can significantly alter the gene expression pattern of genes involved in variety of biological processes and signal transduction pathways. PMID: 25217229
11. There is an association between chemosensitivity and Pgp, GST-pi and Topo II expression in gastric cancer. PMID: 24326092
12. These results are consistent with the hypothesis that etoposide quinone contributes to etoposide-related leukemogenesis through an interaction with topoisomerase IIbeta. PMID: 24766193
13. Downregulating topoisomerase IIbeta confers resistance specifically to mitoxantrone. PMID: 23696245
14. Top2beta-DNA cleavage complexes arrest transcription elongation and induce a proteasomal degradation of Top2beta on DNA. PMID: 23938298
15. results suggest that the low expression of TOPOIIbeta in patients with tongue carcinoma indicates that intrinsic drug resistance may exist in tongue carcinoma, and is associated with tumor differentiation and cisplatin resistance in tongue carcinoma. PMID: 21793937
16. Overexpression of Lewis(y) antigen confers cell adhesion-mediated drug resistance to apoptosis in ovarian cancer cells by the upregulation of Topo-I and Topo-II beta. PMID: 21542140
17. Tyrosine 656 in topoisomerase II beta is important for the catalytic activity of the enzyme. PMID: 21280220
18. [review] Accumulating studies show the significant role of Top IIbeta in neuronal development through regulating expression of certain genes in cells committed to the neuronal fate after the final division. PMID: 20882068
19. shows for the first time that topoisomerase II beta is a substrate for PKC zeta, and that PKC zeta may significantly influence topoisomerase II inhibitor-induced cytotoxicity by altering topoisomerase II beta activity through its kinase function PMID: 12105221
20. analysis of binding sites for NF-Y and Sp1, which are critical for TOP2B transcription PMID: 12197834
21. proteasomal degradation of TOP2beta induced by the TOP2-DNA covalent complex or the TOP2 circular clamp is due to transcriptional arrest but not DNA damage PMID: 12629207
22. Topoisomerase II is a potential target for new antilesishmanial drug development. PMID: 14723357
23. When both topo IIalpha and topo IIbeta were removed, the segregation of chromosomes was severely arrested, suggesting that topo IIbeta could partially substitute for topo IIalpha. PMID: 14996935
24. TOP2A may have a role in response to doxorubicin-based chemotherapy PMID: 16502015
25. reported that signal-dependent activation of gene transcription by nuclear receptors and other classes of transcription factors requires DNA topoisomerase IIbeta-dependent, transient, site-specific dsDNA break formation PMID: 16794079
26. Reduced expression of topo IIbeta induces apoptosis in part by impairing the anti-oxidant capacity of the cell owing to downregulation of PRDX2. PMID: 16932348
27. EGF may have a role in drug resistance in cultured tumor cells through the downregulation of topoII PMID: 16969495
28. directed mutation of TOP2B demonstrated missense mutations selected for acridine resistance varied with acridine structure PMID: 17209120
29. The C-terminal region of human topoisomerase II beta determines its isoform-specific functions in proliferating cells. PMID: 17526531
30. TopoIIbeta binds a retinoic acid response element. Inhibition causes hyperacetylation of histone 3 at lysine 9 & transcription activation.Increased levels of & association with TopoIIbeta cause resistance to RA in acute promyelocytic leukemia cell lines. PMID: 18212063
31. NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1.DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex. PMID: 19181665
顯示更多

收起更多
相關(guān)疾病：

A defect in TOP2B is associated with autism spectrum disorder (ASD).
亞細胞定位：

Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus.
蛋白家族：

Type II topoisomerase family
組織特異性：

Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung. Also found in breast, colon and lung carcinomas, Hodgkin's disease, large-cell non-Hodgkin's lymphoma, lymphocytic lymphomas and seminomas.
數(shù)據(jù)庫鏈接：

HGNC: 11990

OMIM: 126431

KEGG: hsa:7155

STRING: 9606.ENSP00000396704

UniGene: Hs.475733