Recombinant Human AP-2 complex subunit beta (AP2B1), partial

中文名稱：

Recombinant Human AP-2 complex subunit beta(AP2B1) ,partial
貨號：

CSB-YP001871HU
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Human AP-2 complex subunit beta(AP2B1) ,partial
貨號：

CSB-EP001871HU
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Human AP-2 complex subunit beta(AP2B1) ,partial
貨號：

CSB-EP001871HU-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Human AP-2 complex subunit beta(AP2B1) ,partial
貨號：

CSB-BP001871HU
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Human AP-2 complex subunit beta(AP2B1) ,partial
貨號：

CSB-MP001871HU
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

AP2B1
Uniprot No.：

P63010
別名：

Adapter related protein complex 2 beta 1 subunit; Adapter-related protein complex 2 beta subunit; Adaptin, beta 2 (beta); Adaptor protein complex AP-2 subunit beta; Adaptor related protein complex 2, beta 1 subunit; ADTB2; AP-2 complex subunit beta; AP105B; AP2 BETA; Ap2b1; AP2B1_HUMAN; Beta adaptin; Beta-2-adaptin; Beta-adaptin; Beta2 adaptin; CLAPB1; Clathrin assembly protein complex 2 beta large chain; Clathrin associated/assembly/adaptor protein, large, beta 1; DKFZp781K0743; Plasma membrane adaptor HA2/AP2 adaptin beta subunit
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
基因功能參考文獻：
1. this study determined a structure of AP2 that includes the clathrin-binding beta2 hinge. PMID: 25061211
2. Results suggest that autosomal recessive hypercholesterolemia (ARH) and beta-arrestin depend on a privileged AP2beta2 appendage site for proper cargo recruitment to clathrin bud sites. PMID: 18843039
3. multiple interactions between PIPKI gamma-p90 and AP-2 lead to spatiotemporally controlled PI(4,5)P(2) synthesis during clathrin-mediated synaptic vesicle endocytosis PMID: 19903820
4. interactions of the EGF receptor with AP-2 mediated by the receptor 974YRAL and di-leucine motifs may contribute to beta2 tyrosine phosphorylation PMID: 12900408
5. AP2 regulates thrombin receptor constitutive internalization and endothelial cell resensitization. PMID: 16581796
6. Clathrin, which interacts with the AP2beta-appendage, achieves ligand displacement in vivo by self-polymerisation as the coated pit matures. PMID: 16903783
7. EGFR, fibrillin-2, P-cadherin and AP2beta as biomarkers for rhabdomyosarcoma diagnostics. PMID: 19469909
顯示更多

收起更多
亞細胞定位：

Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
蛋白家族：

Adaptor complexes large subunit family
組織特異性：

Expressed in the brain (at protein level).
數據庫鏈接：

HGNC: 563

OMIM: 601025

KEGG: hsa:163

STRING: 9606.ENSP00000314414

UniGene: Hs.514819