Recombinant Escherichia coli Ribosomal large subunit pseudouridine synthase F (rluF)

中文名稱：

大腸桿菌rluF重組蛋白
貨號：

CSB-YP329191ENV
規格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌rluF重組蛋白
貨號：

CSB-EP329191ENV
規格：
來源：

E.coli
其他：

中文名稱：

大腸桿菌rluF重組蛋白
貨號：

CSB-EP329191ENV-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌rluF重組蛋白
貨號：

CSB-BP329191ENV
規格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌rluF重組蛋白
貨號：

CSB-MP329191ENV
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

rluF
Uniprot No.：

P32684
別名：

rluF; yjbC; b4022; JW3982; Dual-specificity RNA pseudouridine synthase RluF; EC 5.4.99.-; EC 5.4.99.21; 23S rRNA pseudouridine(2604) synthase; Ribosomal large subunit pseudouridine synthase F; rRNA pseudouridylate synthase F; rRNA-uridine isomerase F; tRNA(Tyr) pseudouridine(35) synthase
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-290
氨基酸序列

MLPDSSVRLN KYISESGICS RREADRYIEQ GNVFLNGKRA TIGDQVKPGD VVKVNGQLIE PREAEDLVLI ALNKPVGIVS TTEDGERDNI VDFVNHSKRV FPIGRLDKDS QGLIFLTNHG DLVNKILRAG NDHEKEYLVT VDKPITEEFI RGMSAGVPIL GTVTKKCKVK KEAPFVFRIT LVQGLNRQIR RMCEHFGYEV KKLERTRIMN VSLSGIPLGE WRDLTDDELI DLFKLIENSS SEVKPKAKAK PKTAGIKRPV VKMEKTAEKG GRPASNGKRF TSPGRKKKGR
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA and from uracil-35 in the anticodon of tRNA(Tyr). Can, to a small extent, also react with uracil-2605.
基因功能參考文獻：
1. Analysis of total tRNA isolated from E. coli pseudouridine synthase knock-out mutants identified RluF as the enzyme responsible for this modification. PMID: 27551044
2. Results describe the crystal structure of an RluF-RNA complex, and suggest that a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome. PMID: 19298824
蛋白家族：

Pseudouridine synthase RsuA family
數據庫鏈接：

KEGG: ecj:JW3982

STRING: 316385.ECDH10B_4211