Recombinant Defective pharyngeal development protein 4 (pha-4), partial

中文名稱：

秀麗隱桿線蟲pha-4重組蛋白
貨號：

CSB-YP619135CXY
規(guī)格：
來源：

Yeast
其他：

中文名稱：

秀麗隱桿線蟲pha-4重組蛋白
貨號：

CSB-EP619135CXY
規(guī)格：
來源：

E.coli
其他：

中文名稱：

秀麗隱桿線蟲pha-4重組蛋白
貨號：

CSB-EP619135CXY-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

秀麗隱桿線蟲pha-4重組蛋白
貨號：

CSB-BP619135CXY
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

秀麗隱桿線蟲pha-4重組蛋白
貨號：

CSB-MP619135CXY
規(guī)格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

pha-4
Uniprot No.：

Q17381
別名：

pha-4; fkh-1; F38A6.1; Defective pharyngeal development protein 4; Ce-fkh-1; Fork head-HNF-3 homolog
種屬：

Caenorhabditis elegans
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts as a transcription factor required for formation of the pharyngeal primordium. Binds to the consensus sequence 5'-T[AG]TT[TG][AG][TC]-3' with variations of the sequence affecting onset of target gene expression. Activates a wide array of pharyngeal genes including ceh-22 and myo-2 and represses ectodermal genes lin-26 and elt-3, ensuring pharyngeal cell fate. Required for recruitment of htz-1 to a subset of pharyngeal promoters to ensure gene activation and also acts synergistically with tbx-2 in pharyngeal development. Acts in the regulation of diet-restriction-mediated longevity by increasing expression of sod-1, sod-2, sod-4 and sod-5 but not sod-3. Positively regulates the expression of the transcription factor hlh-6 in the pharyngeal gland. May regulate gene expression in the intestine.
基因功能參考文獻：
1. results suggest that PHA-4 employs feed-forward loops to fine-tune gene expression and instil robustness in the system during energy crisis. PMID: 25504288
2. these results suggest that Pol II recruitment, in addition to chromatin opening, is an important feature of PHA-4 pioneer factor activity. PMID: 26089518
3. PHA-4 binding and activation of pharyngeally expressed promoters was restricted to a subset of pharyngeal cells and excluded from the intestine. PMID: 20714352
4. features of PHA-4 response elements PMID: 20623595
5. Thousands of binding sites for PHA-4 during formation of the embryonic pharynx and a role for this factor during the starvation response, were identified. PMID: 20174564
6. data suggest that PHA-4 and DAF-12 endow the pharynx with transcriptional plasticity to respond to diverse developmental and physiological cues PMID: 15375261
7. The transcription factor PHA-4, functions together with various cis-regulatory elements in target genes to regulate spatial and temporal patterning during development of the pharynx. PMID: 15693952
8. the PHA-4 amino terminus is essential for PHA-4 function in vivo, possibly as a transactivation domain, and can compensate for loss of the carboxyl terminus PMID: 16127716
9. restriction of early blastomeres to the pharyngeal fate depends on both repression of ectodermal genes and activation of pharyngeal genes by PHA-4 PMID: 17250823
10. role of PHA-4 in lifespan determination is specific for dietary restriction, because it is not required for the increased longevity caused by other genetic pathways that regulate ageing PMID: 17476212
11. C. elegans pha-4 encodes a FoxA transcription factor that is required to establish the foregut in embryos and to control growth and longevity after birth. PMID: 18804378
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亞細胞定位：

Nucleus.
組織特異性：

Strongly expressed in the embryonic pharynx, particularly pharyngeal primordium precursor cells. Expression less strong in the embryonic gut. Expressed in adult intestine.
數(shù)據(jù)庫鏈接：

KEGG: cel:CELE_F38A6.1

STRING: 6239.F38A6.1a

UniGene: Cel.17389