BUB1B Recombinant Monoclonal Antibody

說明書

中文名稱：

BUB1B重組抗體
貨號：

CSB-RA263021A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate
All lanes: BubR1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 120, 106, 122 kDa
Observed band size: 125 kDa

Overlay histogram showing Hela cells stained with CSB-RA263021A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA263021A0HU BUB1B重組單克隆抗體是針對紡錘體檢查點關鍵調控因子BUB1B（BUB1 Mitotic Checkpoint Serine/Threonine Kinase B）開發的高特異性科研試劑。該抗體通過重組表達技術制備，經ELISA、蛋白質印跡（WB）和流式細胞術（FC）等多平臺驗證，在WB實驗中推薦使用1:500-1:5000的寬泛稀釋范圍，FC應用建議采用1:20-1:200的稀釋比例，展現出優異的批次穩定性和實驗重復性。BUB1B作為維持染色體分離準確性的核心蛋白，其功能研究對解析細胞周期調控機制及基因組穩定性具有重要意義。本產品可廣泛應用于有絲分裂過程分析、腫瘤細胞增殖模型構建以及DNA損傷應答研究等領域，特別適用于探究細胞周期檢查點異常導致的染色體錯誤分離機制。通過優化的抗原表位設計，該抗體能精準識別天然構象及變性狀態下的靶蛋白，為細胞生物學研究提供高靈敏度的檢測工具，滿足體外實驗中對BUB1B蛋白表達水平及亞細胞定位的分析需求。
Uniprot No.：

O60566
基因名：

BUB1B
別名：

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC 2.7.11.1) (MAD3/BUB1-related protein kinase) (hBUBR1) (Mitotic checkpoint kinase MAD3L) (Protein SSK1), BUB1B, BUBR1 MAD3L SSK1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human BubR1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7H4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
基因功能參考文獻：
1. Human gastric cancer tissues with low BUBR1 expression showed no eNOS expression. A decrease in BUBR1 reduced eNOS bioavailability through a pathway other than eNOS phosphorylation. PMID: 30396924
2. Authors show that two distinct pools of BubR1/Bub3 exist at kinetochores and we uncouple these with defined BubR1/Bub3 mutants to address their function. PMID: 27457023
3. we found that FOXM1 inhibitor attenuated tumorigenesis and radioresistance of glioblastoma (GBM) both in vitro and in vivo. Altogether, BUB1B promotes tumor proliferation and induces radioresistance in GBM, indicating that BUB1B could be a potential therapeutic target for GBM. PMID: 29039578
4. Results from phylogenomic study identified of a novel conserved cassette of short linear motifs in BubR1 essential for the spindle checkpoint PMID: 28003474
5. BubR1 N-terminal domain was necessary, but not sufficient to protect against aneuploidy and cancer. In contrast, BubR1 lacking the internal Cdc20-binding domain provided protection against both, which coincided with improved microtubule-kinetochore attachment error correction and spindle assembly checkpoint activity. PMID: 27528194
6. Low BUB1B expression is associated with Chromophobe Renal Cell Carcinomas. PMID: 28807937
7. Whether carriers of pathogenic BUB1B mutations, such as the parents of MVA syndrome patients, have an increased risk for cancer remains of interest, as studies in mice have suggested that haploinsufficiency of BUB1B may cause an increase in carcinogen-induced tumors PMID: 27239782
8. structure of the PP2A B56-BubR1 complex provides important insights into how the B56 subunit directs the recruitment of PP2A to specific targets. PMID: 27350047
9. Overexpression of BUB1B is associated with Invasive Breast Cancer. PMID: 27165245
10. we showed that BubR1 and Mad2 are overexpressed in oral squamous cell carcinoma cell lines and linked such overexpression to attenuated spindle assembly checkpoint activity. We also showed BubR1 overexpression to be associated with advanced stage and tumour size. PMID: 25754611
11. The integrity of the mitotic checkpoint complex depends on the specific recognition between BubR1 and Bub3, for which the BubR1 Gle2 binding sequence motif is essential. PMID: 27030009
12. We show that kinetochore recruitment of BUBR1 and BUB3 by BUB1 is dispensable for SAC activation PMID: 26148513
13. Data suggest that both BubR1 and SNCG may be promising predictive marker rather than prognostic marker in patients with breast cancer. PMID: 26191236
14. BubR1 knockdown significantly decreased cellular invasion but slightly affect cellular proliferation on both Ca9-22 and Cal-27 cells. PMID: 26151845
15. BubR1 contributes to preventing premature aging. [review] PMID: 25964054
16. Suggest human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy in HPV-induced cervical cancer. PMID: 25789401
17. In conclusion, the results presented here suggest that Mad2 and BubR1 could be used as prognostic markers of tumor progression and new pharmacological targets in the treatment for gastric cancer . PMID: 25483095
18. Co-depletion of MAD2 and BUBR1 causes cell cycle arrest and cell death in addition to aneuploidy. PMID: 24687487
19. By sequestering PIDD at the kinetochore, BubR1 acts to delay PIDDosome formation until the next cycle, defining a new mechanism by which cells evade apoptosis during mitosis. PMID: 25936804
20. a Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing PMID: 25482201
21. study shows that SIRT2 is a deacetylase for BubR1 K250, although the abnormally prolonged SAC activation observed in SIRT2 knockdown cells is not accompanied by a change in BubR1 levels or by delayed progression from prometaphase to anaphase PMID: 25285631
22. YAP constitutively associated with BubR1 (BUB1-related protein kinase), and knockdown of BubR1 relieved YAP-driven hyperactivation of the spindle checkpoint. PMID: 25605730
23. Data indicate that cell cycle protein Bub3-mediated kinetochore recruitment of BubR1 kinase enhances mitotic checkpoint signaling. PMID: 25246557
24. the BubR1M-Cdc20 interaction indirectly contributes to mitotic checkpoint complex homeostasis PMID: 25505175
25. The ABBA motif in cyclin A is required for its proper degradation in prometaphase through competing with BUBR1 for the same site on CDC20 PMID: 25669885
26. Our findings indicated an interplay between BUBR1 and p53 in colorectal cancer. Altered expression of both molecules was associated with chromosomal instability. PMID: 25275037
27. the loss of BubR1 levels with age is due to a decline in NAD(+) and the ability of SIRT2 to maintain lysine-668 of BubR1 in a deacetylated state, which is counteracted by the acetyltransferase CBP. PMID: 24825348
28. In acute myeloid leukemia, repression of BubR1 is associated with enhanced anaphase-promoting complex activity. PMID: 23812934
29. p53 deficiency may lead to the failure of BubR1 downregulation by OS and that p53 deficiency and BubR1 accumulation could contribute to gastric carcinogenesis associated with aneuploidy. PMID: 24156017
30. BubR1 overexpression was associated with cell proliferation and may play a role in the carcinogenesis of gastrointestinal diffuse large B cell lymphoma PMID: 23400934
31. These results suggest that the malignant transformation of plexiform neurofibroma is associated with distinct changes in the expression of BUB1B, PBK and NEK2 PMID: 23370767
32. High levels of BubR1 were less sensitive to the anti-microtubule drugs paclitaxel and nocodazole in esophageal squamous cell carcinoma. PMID: 23128493
33. Data suggest that BubR1 counteracts Aurora B kinase activity at improperly attached kinetochores by recruiting B56-PP2A phosphatase complexes. PMID: 23345399
34. Reduced BubR1 expression is strongly associated with longer survival in prostate cancer patients. PMID: 23475578
35. Mad2 Binding Induces a Functional Switch in Cdc20,Enabling BubR1 Binding. PMID: 23791783
36. findings highlight the insufficiency of BUB1 haploinsufficiency to directly stimulate tumourigenesis, and suggest that other factors may be more critical to this process. PMID: 23440991
37. Rsf-1 increases the frequency of abnormal mitotic events by disrupting hBubR1-Cdc20 interactions. PMID: 23536579
38. Results suggest that targeting the GLEBS domain activity of BUB1B may provide a therapeutic window for glioblastoma. PMID: 23154965
39. Elevated BUBR1 expression was associated with poor survival in early stage breast cancer patients PMID: 23392733
40. The PLK1 and BUBR1 cooperate to stabilize kinetochore-microtubule interactions by regulating PP2A-B56alpha-mediated dephosphorylation of Aurora B substrates at the kinetochore-microtubule interface. PMID: 23079597
41. It was shown that the state of CENP-E-dependent BubR1 autophosphorylation in response to spindle microtubule capture by CENP-E is important for kinetochore function in achieving accurate chromosome segregation. PMID: 22801780
42. Results reveal that BubR1 sumoylation plays an important role in its timely removal from the kinetochores and the checkpoint inactivation, thus allowing normal anaphase entry and chromosome segregation. PMID: 22374677
43. It was shown that Mad3/BUBR1 and BUB1 paralogous pairs arose by nine independent gene duplications throughout evolution. It was also shown that putative catalysis by human BUBR1 is dispensable for error-free chromosome segregation. PMID: 22698286
44. p31(comet) negatively regulates the spindle assembly checkpoint by extracting Mad2 from the MCC. PMID: 22100920
45. a new type of post-translational modification that is essential for BubR1 function during mitosis. PMID: 22167194
46. we identify the Blinkin motif critical for interaction with BUBR1, define the stoichiometry and affinity of the interaction, and present a 2.2 Angstrom resolution crystal structure of the complex PMID: 22000412
47. DLGAP5-PINK1 and BUB1B-PINK1 were strong predictors of disease-free survival and overall survival, respectively, among adult patients with ACT. PMID: 22048964
48. Aging-related loss of BubR1 and subsequent impairment of reactivity to reactive oxygen species may explain reduced proliferative capacity of aged smooth muscle cells. PMID: 21550059
49. Our data imply the possibility that BUBR1 may be involved in the progression of oral squamous cell carcinoma, and suggest that BUBR1 may be a promising prognostic marker in patients with OSCC PMID: 21069850
50. BUBR1 and closed MAD2 (C-MAD2) interact directly to assemble a functional mitotic checkpoint complex PMID: 21525009
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相關疾病：

Premature chromatid separation trait (PCS); Mosaic variegated aneuploidy syndrome 1 (MVA1)
亞細胞定位：

Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
組織特異性：

Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
數據庫鏈接：

HGNC: 1149

OMIM: 176430

KEGG: hsa:701

STRING: 9606.ENSP00000287598

UniGene: Hs.513645

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