Phospho-PLK4 (S305) Antibody
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中文名稱：磷酸化-PLK4 (S305)兔多克隆抗體
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貨號：CSB-PA018196XA01phHU
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規格：￥440
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Serine/threonine-protein kinase PLK4Curated (EC:2.7.11.213 Publications), Polo-like kinase 4, PLK-4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak, PLK4Imported, SAK, STK18
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反應種屬：Homo sapiens (Human)
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免疫原：Synthesized peptide derived from Human PLK4 around the phosphorylation site of S305
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Affinity-purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA
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Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
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靶點詳情
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功能：Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification. Phosphorylates CEP131 at 'Ser-78' and PCM1 at 'Ser-372' which is essential for proper organization and integrity of centriolar satellites.
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基因功能參考文獻：
- Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly. PMID: 29712910
- KAT2A/2B acetylation of PLK4 prevents centrosome amplification PMID: 27796307
- PLK4 played important roles in regulating cell cycle- and DNA replication-related pathways. E2F could upregulate the expression levels of PLK4 by deregulating the methylations of their promoters to promote the relapse of Acute Lymphoblastic Leukemia. PMID: 29768346
- PLK4 specifically phosphorylates CP110 at the S98 position and is an essential step for centriole assembly. PMID: 28562169
- These data show that complementary mechanisms, such as mother-daughter centriole proximity and CDK1-CyclinB interaction with centriolar components, ensure that centriole biogenesis occurs once and only once per cell cycle, raising parallels to the cell-cycle regulation of DNA replication and centromere formation. PMID: 27112295
- Homozygous splicing acceptor site transition (c.31-3 A>G) in PLK4 was identified in a family with Seckel syndrome. PLK4 is essential for centriole biogenesis and DNA damage response. PMID: 28832566
- the interaction between Cep78 and the N-terminal catalytic domain of Plk4 is a new and important element in the centrosome overduplication process. PMID: 27246242
- Our results validate Plk4 as a therapeutic target in cancer patients PMID: 27872092
- Heterozygous missense mutation in PLK4 identified in a patient with microcephaly and chorioretinopathy. Aberrant spindle formation was observed in a LCL derived from this patient. Mutant PLK4 proteins demonstrated altered mobility pattern on a western blot suggesting alterations in post-translation modification. PMID: 27650967
- Studies indicate that depletion of any one of the protein kinase polo-like kinase 4 (PLK4) and the two proteins STIL and SAS-6 blocks centriole duplication, and, conversely, overexpression causes centriole amplification. PMID: 27911707
- Common PLK4 variant rs2305957 is associated with blastocyst formation and early recurrent miscarriage in Chinese women. PMID: 28238495
- Mutations in human PLK4, the protein of which plays critical role in centriole duplication and normal nuclear formation, could be associated with abnormal spermatogenesis leading to Sertoli cell-only syndrome. Aberrant forms of PLK4 might also cause other types of oligozoospermia or sperm fl agellar abnormalities. PMID: 26452337
- Plk4 directly binds PCM1 and phosphorylates S372. Plk4 depletion leads to the dispersal of centriolar satellites. PMID: 26755742
- Decreased PLK4 protein expression due to promoter hypermethylation was negatively correlated with JAK2 overexpression, a common occurrence in hematological malignancies. PMID: 25347426
- KLF14 transcription is significantly downregulated, whereas Plk4 transcription is upregulated in multiple types of cancers, and there exists an inverse correlation between KLF14 and Plk4 protein expression in human breast and colon cancers. PMID: 26439168
- The authors suggest that the STIL-coiled-coil region/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication. PMID: 26188084
- We demonstrate that centrioles promote PLK4 activation through its recruitment and local accumulation. Though centriole removal reduces the proportion of active PLK4, this is rescued by concentrating PLK4 to the peroxisome lumen PMID: 26481051
- these results identify the interaction between Mib1 and Plk4 as a new and important element in the control of centriole homeostasis. PMID: 25795303
- PLK4 functions downstream of ROCK2 to drive centrosome amplification in arrested cells. PMID: 25590559
- PLK4 overexpression induces centrosome amplification and chromosome instability and causes the suppression of primary cilia formation. PMID: 24981932
- Negative feedback by centriolar STIL regulates bimodal centriolar distribution of Plk4 and seemingly restricts occurrence of procentriole formation to one site on each parental centriole. PMID: 25342035
- An unexpected activity of Plk4 that promotes cell migration and may underlie an association between increased Plk4 expression, cancer progression and death from metastasis in solid tumor patients. PMID: 25174401
- Plk4 activity promotes the recruitment of STIL to the centriole and primes the direct binding of STIL to the C terminus of SAS6. PMID: 26101219
- Mutation in PLK4, encoding a master regulator of centriole formation, defines a novel locus for primordial dwarfism. PMID: 25320347
- Data suggest polo-like kinase 4 (PLK4) inhibitors as a clinical candidate for cancer therapy. PMID: 25723005
- Studies indicate that overexpression of polo-like kinase 4 (PLK4) is found in several cancer and suggest the PLK4 inhibitors as anticancer therapeutics. PMID: 24867403
- identified association between aneuploidy of putative mitotic origin and linked genetic variants on chromosome 4 of maternal genomes; associated region contains candidate gene,PLK4, that plays a role in centriole duplication and can alter mitotic fidelity upon minor dysregulation PMID: 25859044
- different levels of impaired PLK4 activity result in growth and cilia phenotypes, providing a mechanism by which microcephaly disorders can occur with or without ciliopathic features PMID: 25344692
- Results demonstrated that Plk4 is under the direct control of the E2F activators in breast cancer cells. PMID: 24797070
- p53-Dependent and cell specific epigenetic regulation of the polo-like kinases under oxidative stress. PMID: 24498222
- Breast cancer cell viability is dependent on PLK4 expression. PMID: 25043604
- Plk4 is intricately regulated in time and space through ordered interactions with two distinct scaffolds, Cep192 and Cep152, and a failure in this process may lead to human cancer. PMID: 24997597
- it appears that Nek2 and Plk4 might synergize to promote breast tumorigenesis and may also be involved in tamoxifen and trastuzumab resistance PMID: 24389189
- PLK4 is a new NFkappaB target gene, providing a direct link between NFkappaB activity and centrosome duplication, with implications for the role of these transcription factors in tumorigenesis. PMID: 23974100
- Plk4 dynamically localizes to distinct subcentrosomal regions by interacting with two hierarchically regulated scaffolds, Cep192 and Cep152. PMID: 24277814
- Studies indicate that autophosphorylation of Nek7 and Plk4 occurred through an intermolecular mechanism, the kinases Aurora-A and Chk2 followed an intramolecular mechanism. PMID: 23821772
- cooperation between Cep192 and Cep152 is crucial for centriole recruitment of Plk4 and centriole duplication during the cell cycle. PMID: 23641073
- If both p53 and the SAPKK MKK4 are simultaneously inactivated, persistent polo-like kinase 4 activity combined with the lack of SAPK-mediated inhibition of centrosome duplication conspire to induce supernumerary centrosomes under stress. PMID: 23653187
- Preventing Plk4 autoregulation causes centrosome amplification, stabilization of p53, and loss of cell proliferation PMID: 23249732
- CAND1 promotes PLK4-mediated centriole overduplication and is frequently disrupted in prostate cancer. PMID: 23019411
- Study demonstrated that PLK4 was remarkably downregulated in HCC and could be served as a potential prognostic marker for patients with this deadly disease. PMID: 22829937
- STIL cooperates with SAS-6 and PLK4 in the control of centriole number and represents a key centriole duplication factor in human cells. PMID: 22349698
- Plk4 is a centriole-localized kinase that does not directly regulate cytokinesis. PMID: 22456511
- The activity of SCF-FBXW5 is negatively regulated by Polo-like kinase 4 (PLK4), which phosphorylates FBXW5 at Ser 151 to suppress its ability to ubiquitylate HsSAS-6. PMID: 21725316
- These results highlight the critical role of PLK4 transcriptional deregulation in centriole multiplication in HPV-16 E7-expressing cells. PMID: 21609466
- CDK11(p58), which accumulates only in the vicinity of mitotic centrosomes, directly interacts with the centriole-associated protein kinase Plk4 that regulates centriole number in cells. PMID: 21297952
- Results suggest that Cep152 recruits Plk4 and CPAP to the centrosome to ensure a faithful centrosome duplication process. PMID: 21059844
- Data show that Cep152 can be phosphorylated by Plk4 in vitro, suggesting that Cep152 acts with Plk4 to initiate centriole formation. PMID: 21059850
- Data suggest that active Plk4 promotes its own degradation by catalyzing betaTrCP binding through trans-autophosphorylation (phosphorylation by the other kinase in the dimer) within homodimers. PMID: 20516151
- Data suggest that polo-like kinase 4 activity is restricted to the centrosome to prevent aberrant centriole assembly and sustained kinase activity is required for centriole duplication. PMID: 20032307
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相關疾病：Microcephaly and chorioretinopathy, autosomal recessive, 2 (MCCRP2)
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亞細胞定位：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus, nucleolus. Cleavage furrow. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
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蛋白家族：Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily
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