Rat pigment epithelium-derived factor,PEDF ELISA Kit

產品詳情

文獻引用 (10) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠色素上皮衍生因子(PEDF)酶聯免疫試劑盒（CSB-E08819r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的PEDF含量。PEDF（色素上皮衍生因子）是一種具有多種生物功能的分泌性糖蛋白，主要在視網膜色素上皮細胞中表達。它具有抗血管生成、抗炎和神經營養作用，對多種腫瘤細胞具有抑制作用。其研究機制涉及通過調節細胞增殖、凋亡和遷移等途徑抑制腫瘤生長。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于體外培養細胞分泌功能評估、疾病模型動物血清標志物分析以及組織微環境中PEDF動態變化的科研探索，為血管生成調控、氧化應激機制及神經退行性疾病等基礎研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PEDF
Uniprot No.：

Q80ZA3
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	87
	Range %	81-92
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	92
	Range %	85-97
1:8000	Average %	96
	Range %	91-102

回收率：

The recovery of rat PEDFspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	87	83-91

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.