Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

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中文名稱：

大鼠核因子-κB亞基p65親和肽(NF-κB p65)酶聯免疫試劑盒
貨號：

CSB-E08788r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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引用文獻

產品描述：

大鼠核因子-κB亞基p65親和肽(NF-κB p65)酶聯免疫試劑盒（CSB-E08788r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NFκB p65含量。NFκB p65是重要的轉錄因子，在細胞的炎癥、免疫、增殖和凋亡等多種生理病理過程中發揮關鍵作用。其激活機制多樣，與多條信號通路相互關聯。對其研究有助于深入了解疾病發病機制，為開發針對性療法如抗炎、抗腫瘤藥物等提供理論基礎。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于基礎科研中炎癥機制研究、免疫調控實驗或藥物干預效果評估，例如在動物模型中分析NF-κB信號通路激活狀態，或探究氧化應激、感染性疾病等實驗條件下p65亞基的動態表達。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

NF-Kappa B,nuclear factor-kappa B
縮寫：

NFκB p65
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	86-92
1:200	Average %	99
	Range %	97-103
1:400	Average %	89
	Range %	85-93
1:800	Average %	95
	Range %	91-99

回收率：

The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.039	2.022	2.031	1.887
50	1.334	1.326	1.330	1.186
25	0.908	0.902	0.905	0.761
12.5	0.566	0.557	0.562	0.418
6.25	0.358	0.364	0.361	0.217
3.12	0.291	0.281	0.286	0.142
1.56	0.243	0.232	0.238	0.094
0	0.145	0.143	0.144

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Targeting TLR4/NF-κB signaling, oxidative stress, and apoptosis by farnesol mitigates cadmium-induced testicular toxicity in rats EHM Hassanein, MF Alotaibi, RS Alruhaimi, M Sabry,Tissue & Cell,2025
Swertiamarin ameliorates cognitive dysfunction by improving hyperglycemia and neuroinflammation in type 2 diabetic rats via activation of the PI3K/AKT/GSK3β signaling pathway B Tan, L Liu, T Wu, F Yuan, C Wang,Central European Journal of Immunology,2024
The sesquiterpene alcohol farnesol mitigates cadmium hepatotoxicity by attenuating oxidative stress and NF-κB/NLRP3 inflammasome axis and upregulating PPARγ in rats RS Alruhaimi, EHM Hassanein, SM Alnasser,EXCLI Journal,2024
Green Synthetized Selenium Nanoparticles Using Syzygium aromaticum (Clove) Extract Reduce Pentylenetetrazol-Induced Epilepsy and Associated Cortical Damage in Rats MS Othman,Applied Sciences,2023
Rosmarinic acid mitigates chlorpyrifos-induced oxidative stress, inflammation, and kidney injury in rats by modulating SIRT1 and Nrf2/HO-1 signaling MS Abduh,Life sciences,2022
Hydrogen gas ameliorates the LPS-induced BPD via inhibiting the activation of TNF-α/NF-κB inflammatory signaling pathway in placenta Y Zhang,researchsquare,2022
Reno-protective effect of mangiferin against methotrexate-induced kidney damage in male rats: PPARγ-mediated antioxidant activity SM Elshazly,Saudi Pharmaceutical Journal,2022
Apigenin attenuates molecular, biochemical, and histopathological changes associated with renal impairments induced by gentamicin exposure in rats Y Xie,ResearchSquare,2022
Inosine mitigated diabetic peripheral neuropathy via modulating GLO1/AGEs/RAGE/NF-κB/Nrf2 and TGF-β/PKC/TRPV1 signaling pathways NF Abdelkader,Biomedecine & pharmacotherapie,2021
Postconditioning attenuates myocardial ischemia?reperfusion injury by inhibiting complement activation and upregulation of miR?499 Z Huang,Experimental and Therapeutic Medicine,2021
Alogliptin attenuates lipopolysaccharide-induced neuroinflammation in mice through modulation of TLR4/MYD88/NF-κB and miRNA-155/SOCS-1 signaling pathways Ayman E El-Sahar,International Journal of Neuropsychopharmacology,2020
Suppression of inflammatory cascades via novel cinnamic acid nanoparticles in acute hepatitis rat model EA Ibrahim,Archives of Biochemistry and Biophysics,2020
Regulation of liver regeneration by prostaglandin E 2 and thromboxane A 2 following partial hepatectomy in rats YS Mohamed,Naunyn-Schmiedeberg\'s Archives of Pharmacology,2020
Vitamin D3 Modulates NF-kB/p65, 17β-Estradiol, and Vitamin D Receptors Expression at Estrogen Deficiency Koshkina A, et al,Vitamin D Defjciency,2019
Inflammatory Consequences of Maternal Diabetes on the Offspring Brain: a Hippocampal Organotypic Culture Study Glombik K,Neurotoxicity Research,2019
Hepato-protective Effect of Ultrasonicated Ginseng Berry Extract on Rat Mild Bile Duct Ligation Model Yoonjin Nam.et al,Journal of Ginseng Research,2018
Involvement of the NF-魏B signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model Shujuan Qiu.et al,Biomedical Reports,2016

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.