Rat low density lipoprotein,LDL ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠低密度脂蛋白(LDL)酶聯(lián)免疫試劑盒（CSB-E11704r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LDL含量。LDL（低密度脂蛋白）是一種運(yùn)載膽固醇進(jìn)入外周組織細(xì)胞的脂蛋白顆粒。其水平升高易導(dǎo)致膽固醇在血管壁沉積，引發(fā)動(dòng)脈粥樣硬化等心血管疾病。研究機(jī)制常聚焦于調(diào)節(jié)其代謝過程，如促進(jìn)其清除、抑制合成等，以降低心血管病風(fēng)險(xiǎn)。試劑盒檢測(cè)范圍為0.312 μg/mL-20 μg/mL，適用于心血管疾病機(jī)制研究、藥物干預(yù)效果評(píng)價(jià)及脂代謝相關(guān)實(shí)驗(yàn)，例如高脂飼料誘導(dǎo)的動(dòng)脈硬化模型評(píng)估、降脂藥物篩選或代謝綜合征動(dòng)物模型中的脂蛋白動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

LDL
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.156 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	93-103
1:200	Average %	104
	Range %	100-108
1:400	Average %	87
	Range %	80-94
1:800	Average %	97
	Range %	94-100

回收率：

The recovery of rat LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	92	88-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.