Rat Thromboxane B2,TXB2 ELISA Kit

Rat Thromboxane B2,TXB2 ELISA Kit

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中文名稱：

大鼠血栓素B2(TXB2)酶聯免疫試劑盒
貨號：

CSB-E08047r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠血栓素B2(TXB2)酶聯免疫試劑盒（CSB-E08047r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TXB2含量。TXB2是一種由TXA2酶促代謝產生的穩定水解產物，是TXA2的穩定代謝物，可以反映TXA2的全身生物合成速率。TXB2在生理和病理過程中扮演重要角色，如參與止血、動脈粥樣硬化、血栓形成等。研究TXB2有助于了解相關疾病的發生機制，為疾病診斷和治療提供新的靶點。試劑盒檢測范圍為3.9 pg/mL-250 pg/mL，適用于心血管疾病動物模型研究、抗血小板藥物藥效評估、炎癥相關機制探索等科研領域，滿足基礎研究中對TXB2動態變化的定量分析需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TXB2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.9 pg/mL-250 pg/mL
靈敏度：

0.97 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-98
1:2	Average %	95
	Range %	91-102
1:4	Average %	99
	Range %	94-106
1:8	Average %	93
	Range %	87-97

回收率：

The recovery of rat TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-103
EDTA plasma (n=4)	92	88-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
250	1.990	1.890	1.940	1.785
125	1.298	1.198	1.248	1.093
62.5	0.786	0.766	0.776	0.621
31.25	0.545	0.525	0.535	0.380
15.6	0.341	0.321	0.331	0.176
7.8	0.292	0.272	0.282	0.127
3.9	0.215	0.211	0.213	0.058
0	0.156	0.154	0.155

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.