Rat Platelet-derived growth factor CC(PDGF-CC) ELISA kit

文獻引用 (1) 說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠血小板衍生生長因子CC(PDGF-CC) 酶聯(lián)免疫試劑盒（CSB-EL017710RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-CC含量。PDGF - CC即血小板衍生生長因子CC，是PDGF家族成員。它在胚胎發(fā)育、組織修復等過程有重要作用。其異常表達與腫瘤、纖維化等疾病相關(guān)。研究機制多圍繞其與受體結(jié)合，激活下游信號通路，影響細胞增殖、遷移等方面。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領(lǐng)域?qū)Υ笫竽Ｐ椭蠵DGF-CC蛋白表達的定量分析，例如在心血管疾病、組織再生或腫瘤機制研究中評估目標分子動態(tài)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-CC
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-95
1:2	Average %	103
	Range %	99-112
1:4	Average %	98
	Range %	94-102
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of rat PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-93
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.