Rat Fatty-acid amide hydrolase 1(FAAH) ELISA kit

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產品詳情

產品描述：

大鼠脂肪酸酰胺水解酶1(FAAH)酶聯(lián)免疫試劑盒（CSB-EL007938RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FAAH含量。FAAH即脂肪酸酰胺水解酶，是一種能降解內源性大麻素的酶。其背景在于它在體內的內源性大麻素系統(tǒng)中發(fā)揮關鍵作用。研究機制主要聚焦在通過抑制FAAH活性，提高內源性大麻素水平，以探索在鎮(zhèn)痛、抗炎、治療精神疾病等方面的應用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒適用于科研領域中對FAAH表達動態(tài)的定量分析，例如探究神經退行性疾病模型、代謝綜合征或炎癥相關動物實驗中FAAH的生物學作用，也可用于評估藥物或化合物對FAAH活性的調控效果。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

Faah; Faah1; Fatty-acid amide hydrolase 1; Anandamide amidase; Anandamide amidohydrolase 1; Fatty acid ester hydrolase; Oleamide hydrolase 1
縮寫：

FAAH
Uniprot No.：

P97612
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FAAH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	107
	Range %	103-110
1:8	Average %	89
	Range %	85-94

回收率：

The recovery of rat FAAH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids.
亞細胞定位：

Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein. Note=Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus.
蛋白家族：

Amidase family
組織特異性：

Found in neuronal cells throughout the CNS. Expressed in liver and brain, and to a lesser extent in spleen, lung, kidney and testes.
數(shù)據(jù)庫鏈接：

KEGG: rno:100911581

STRING: 10116.ENSRNOP00000015961

UniGene: Rn.89119