Rat Complement 4,C4 ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠補體蛋白4(C4)酶聯免疫試劑盒（CSB-E08706r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4含量。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于體外免疫學研究，如炎癥動物模型中的血清C4動態監測、組織局部補體激活水平評估，或藥物干預下補體通路的功能研究，為免疫學、病理學及藥理學領域的科研工作提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C4 ELISA Kit; C4aComplement C4 [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA Kit
縮寫：

C4
Uniprot No.：

P08649
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	99
	Range %	96-102
1:400	Average %	87
	Range %	82-93
1:800	Average %	100
	Range %	96-104
1:1600	Average %	84
	Range %	81-89

回收率：

The recovery of rat C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	88	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory processes. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
基因功能參考文獻：
1. Our study provides the first evidence that sustained selective EPAC activation leads to an increase in TRPC3 and C4 protein expression and induces a proarrhythmic SOCE-like activity in adult rat ventricular cardiomyocytes PMID: 26219954
2. The expression of the complement component C3f and complement component 4A have potential for use in post-transplant diagnosis of rejection. PMID: 23613499
3. This study presents the genomic organization of the two C4 gene-containing modules and the expression of the two C4 genes in the rat (Rattus norvegicus). PMID: 15634920
亞細胞定位：

Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
數據庫鏈接：

KEGG: rno:24233

STRING: 10116.ENSRNOP00000037902

UniGene: Rn.155573