Rat Collagen Type Ⅳ,Col Ⅳ ELISA KIT

產品詳情

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文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠Ⅳ型膠原(Col Ⅳ)酶聯免疫試劑盒（CSB-E08883r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅳ含量。Col Ⅳ即Ⅳ型膠原，是基底膜的主要成分。它在維持組織正常結構與功能中發揮重要作用。研究發現其異常表達與腫瘤侵襲、轉移及多種疾病相關，機制可能涉及與細胞表面受體相互作用，影響細胞黏附、遷移和信號傳導等過程。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，可應用于肝/腎纖維化動物模型構建、血管基底膜損傷研究、腫瘤微環境分析等科研領域，特別適用于評估疾病模型中Col Ⅳ的動態變化及組織損傷修復機制，為心血管疾病、器官纖維化等病理過程的分子機制研究提供精準檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
縮寫：

Col Ⅳ
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	96
	Range %	92-101
1:4	Average %	92
	Range %	87-96
1:8	Average %	87
	Range %	82-94

回收率：

The recovery of rat COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	104	99-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.