Mouse Collagen Type Ⅳ,Col Ⅳ ELISA KIT

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠Ⅳ型膠原(Col Ⅳ)酶聯免疫試劑盒（CSB-E08884m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅳ含量。Col Ⅳ，即IV型膠原，是一種主要的細胞外基質蛋白，在多種生理過程中扮演關鍵角色，如血管生成、組織修復和細胞粘附。其研究機制涉及與MMP-12等酶的相互作用，以及通過降解Col-Ⅳ來誘導血管生成等生物學過程。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于科研領域中小鼠模型的相關研究，例如通過檢測血清/血漿中Col Ⅳ濃度評估全身性基底膜代謝狀態，或通過分析肝、腎等組織勻漿的Col Ⅳ表達水平，探究器官特異性纖維化進展及藥物干預效果?？蓾M足體外實驗中對Col Ⅳ動態變化的定量分析需求，為基底膜相關機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
縮寫：

Col Ⅳ
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-103
1:2	Average %	89
	Range %	84-93
1:4	Average %	88
	Range %	84-92
1:8	Average %	101
	Range %	97-106

回收率：

The recovery of mouse COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	96	93-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.