Pig Mucin-2,MUC2 ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬粘蛋白/粘液素2(MUC2)酶聯免疫試劑盒（CSB-E15066p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MUC2含量。MUC2是一種分泌型粘蛋白，主要在小腸和結腸的杯狀細胞中表達。它在維持腸道穩態中發揮重要作用，其破壞與多種疾病和癌癥有關。MUC2的研究涉及其在腸道黏膜屏障損傷中的作用，以及其基因表達調控機制。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，該產品適用于豬腸道黏膜屏障功能研究、消化道疾病模型中的黏液分泌機制探索，以及營養干預對腸道健康影響的評估等科研領域，尤其適合通過多類型樣本（如腸道組織勻漿與體液樣本）對比分析MUC2的動態變化，為豬的腸道生理病理研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

MUC2
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig mucin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	87-98
1:8	Average %	92
	Range %	85-95

回收率：

The recovery of pig mucin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-96
EDTA plasma (n=4)	98	95-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.