Mouse Mucin-2,MUC2 ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠粘蛋白/粘液素2(MUC2)酶聯免疫試劑盒（CSB-E15065m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MUC2含量。MUC2是一種粘蛋白，在多種上皮組織表面發揮保護屏障作用，維持腸道穩態。研究發現其表達異常與腫瘤、炎癥性腸病等疾病相關。機制上，它通過調節細胞黏附、信號傳導等過程影響疾病發生發展，可作為潛在治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，試劑盒適用于腸道炎癥模型研究、黏液分泌機制探索、黏膜屏障功能評估等科研領域，支持體外實驗和動物實驗中的動態監測需求，為消化道疾病機制研究及藥物開發提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Muc2 ELISA Kit; Mucin-2 ELISA Kit; MUC-2 ELISA Kit; Colonic mucin ELISA Kit; MCM ELISA Kit; Secreted gel-forming mucin ELISA Kit; Fragments ELISA Kit
縮寫：

MUC2
Uniprot No.：

Q80Z19
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	90
	Range %	83-97
1:40	Average %	95
	Range %	86-107
1:80	Average %	95
	Range %	86-102
1:160	Average %	95
	Range %	88-103

回收率：

The recovery of mouse MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-103
EDTA plasma (n=4)	93	89-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
亞細胞定位：

Secreted. Note=In the intestine, secreted into the inner and outer mucus layers.
組織特異性：

Highly expressed in goblet cells of the colon with lower levels in the small intestine and no expression in the stomach (at protein level).
數據庫鏈接：

STRING: 10090.ENSMUSP00000026590

UniGene: Mm.2041