Mouse Eotaxin 2 ELISA Kit

Mouse Eotaxin 2 ELISA Kit

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中文名稱：

小鼠嗜酸粒細胞趨化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶聯免疫試劑盒
貨號：

CSB-E07278m
規格：

96T/48T
價格：

￥3200/￥2500
促銷：
其他：

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小鼠嗜酸粒細胞趨化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶聯免疫試劑盒（CSB-E07278m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL24含量。CCL24，又稱嗜酸性粒細胞趨化蛋白2（eotaxin-2）和骨髓祖細胞抑制因子2（MPIF-2），是一種CC趨化因子，位于人類基因組第7號染色體。CCL24通過與其唯一受體CCR3結合，主要介導特應性疾病、寄生蟲感染和全身性疾病，同時促進細胞運輸并調節炎癥和纖維化活動。研究顯示，CCL24在纖維化疾病中發揮重要作用，其表達水平與多種纖維炎癥性疾病相關。試劑盒檢測范圍為15.6 pg/ml- 1000 pg/ml，靈敏度為3.9 pg/ml。為探索炎癥相關疾病的分子機制、評估藥物干預效果或構建疾病模型提供可靠工具；適用于體外培養細胞上清、動物模型體液及組織樣本的CCL24表達水平分析，滿足科研人員對趨化因子網絡調控研究的多元化需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Ccl24 ELISA Kit; Scya24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
縮寫：

CCL24
Uniprot No.：

Q9JKC0
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/ml - 1000 pg/ml
靈敏度：

3.9 pg/ml
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-96
1:2	Average %	94
	Range %	91-105
1:4	Average %	96
	Range %	92-99
1:8	Average %	93
	Range %	86-98

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.612	2.634	2.623	2.507
500	2.200	2.267	2.233	2.117
250	1.453	1.528	1.491	1.374
125	0.753	0.729	0.741	0.625
62.5	0.349	0.329	0.339	0.222
31.2	0.242	0.253	0.248	0.131
15.6	0.180	0.184	0.182	0.066
0	0.113	0.120	0.116

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Protein tyrosine phosphatase 11 acts through RhoA/ROCK to regulate eosinophil accumulation in the allergic airway Xu C, et al,Faseb Journal,2019

功能：

Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
基因功能參考文獻：
1. the increased airway eosinophils and mucous cell metaplasia in house dust mite-challenged Cd163-/- mice are mediated by augmented CCL24 production PMID: 26376364
2. this study shows that TPL-2 inhibits Ccl24 expression in lung dendritic cells, and blockade of Ccl24 prevents the exaggerated airway eosinophilia and lung inflammation in mice given house dust mites-pulsed Map3k8-/- dendritic cells PMID: 27484038
3. production in M-CSF-induced bone marrow-derived macrophages is synergistically induced by IL-4 and IL-10 PMID: 25267883
4. Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
5. Data indicate that eotaxin 2 (CCL-24), which is known to influence eosinophil migration, was highly up-regulated in broncho-alveolar macrophages (BAMs). PMID: 23685352
6. The oesophageal production of CCL24 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
7. Results demonstrate a cooperative mechanism between interleukin-13 and eotaxin-2, where IL-13 mediates allergen-induced eotaxin-2 expression, and eotaxin-2 mediates IL-13-induced airway eosinophilia. PMID: 15647285
8. Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
9. The eotaxin-2 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
10. eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
11. CCL24 seems important for recruitment of eosinophils into BAL. PMID: 18850374
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亞細胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
組織特異性：

Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis.
數據庫鏈接：

KEGG: mmu:56221

STRING: 10090.ENSMUSP00000004936

UniGene: Mm.31505

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.