Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:20

Average %

85

Range %

81-90

1:40

Average %

98

Range %

91-105

1:80

Average %

89

Range %

84-94

1:160

Average %

90

Range %

86-98

The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

102

97-110

EDTA plasma (n=4)

100

98-102

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2500

2.844

2.734

2.789

2.662

1250

2.331

2.197

2.264

2.137

625

1.488

1.528

1.508

1.381

312.5

0.810

0.829

0.819

0.693

156.25

0.546

0.521

0.533

0.407

78

0.357

0.363

0.360

0.234

39

0.221

0.214

0.218

0.091

0

0.128

0.125

0.127