Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of human C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:100

Average %

102

Range %

92-108

1:200

Average %

94

Range %

89-99

1:400

Average %

97

Range %

91-104

1:800

Average %

94

Range %

89-98

The recovery of human C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

89

85-92

EDTA plasma (n=4)

94

90-104

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2500

2.991

3.107

3.049

2.920

1250

2.476

2.457

2.467

2.338

625

1.423

1.363

1.393

1.264

312

0.657

0.643

0.650

0.521

156

0.327

0.329

0.328

0.199

78

0.208

0.202

0.205

0.076

39

0.171

0.163

0.167

0.038

0

0.131

0.127

0.129