Mouse 4-Hydroxynonenal (HNE)ELISA Kit

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中文名稱：

小鼠4-羥基壬烯酸(HNE)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E13412m
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
促銷：
其他：

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠4-羥基壬烯酸(HNE)酶聯(lián)免疫試劑盒（CSB-E13412m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的HNE含量。HNE即人中性粒細(xì)胞彈性蛋白酶，在炎癥反應(yīng)等生理病理過(guò)程中扮演重要角色。其過(guò)度表達(dá)與肺氣腫、急性呼吸窘迫綜合征等疾病密切相關(guān)。研究主要圍繞抑制其活性展開，期望開發(fā)出能治療相關(guān)疾病的藥物，為臨床治療提供新方向。試劑盒檢測(cè)范圍為15.6 pmol/mL-1000 pmol/mL，本試劑盒可應(yīng)用于氧化損傷相關(guān)研究領(lǐng)域，如代謝性疾病模型、心血管功能異常或神經(jīng)退行性病變等科研場(chǎng)景中，通過(guò)檢測(cè)血清或血漿樣本的HNE水平，評(píng)估氧化應(yīng)激程度及其在疾病進(jìn)展中的作用機(jī)制，為實(shí)驗(yàn)動(dòng)物模型的病理機(jī)制探索提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

HNE
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測(cè)范圍：

15.6 pmol/mL-1000 pmol/mL
靈敏度：

3.9 pmol/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	96
	Range %	91-103
1:4	Average %	94
	Range %	90-99
1:8	Average %	94
	Range %	88-98

回收率：

The recovery of mouse HNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-97
EDTA plasma (n=4)	94	90-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	Corrected
1000	2.608	2.555	2.582	2.392
500	1.939	1.897	1.918	1.728
250	1.227	1.212	1.220	1.030
125	0.821	0.759	0.790	0.600
62.5	0.468	0.450	0.459	0.269
31.2	0.331	0.328	0.330	0.140
15.6	0.278	0.271	0.275	0.085
0	0.192	0.188	0.190