Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit
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中文名稱：人細(xì)胞間粘附分子3(ICAM-3/CD50)酶聯(lián)免疫試劑盒
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貨號：CSB-E07949h
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規(guī)格：96T/48T
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價(jià)格：￥3200/￥2500
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促銷：
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人細(xì)胞間粘附分子3(ICAM-3/CD50)酶聯(lián)免疫試劑盒（CSB-E07949h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿,組織勻漿樣本中的ICAM3含量。ICAM3，即細(xì)胞間粘附分子3，是一種跨膜糖蛋白，屬于ICAM家族，主要功能是介導(dǎo)細(xì)胞間的粘附，參與免疫反應(yīng)和細(xì)胞信號轉(zhuǎn)導(dǎo)。其研究機(jī)制涉及ICAM3與LFA-1等受體的相互作用，以及其在炎癥反應(yīng)和腫瘤浸潤中的作用。試劑盒檢測范圍為31.25 pg/ml- 2000 pg/ml，靈敏度為7.8 pg/ml。為研究免疫相關(guān)疾病機(jī)制、炎癥模型建立、藥物靶點(diǎn)驗(yàn)證等科研需求提供可靠工具，在自身免疫性疾病研究、感染免疫學(xué)實(shí)驗(yàn)及免疫調(diào)節(jié)劑開發(fā)等領(lǐng)域具有廣泛應(yīng)用潛力，滿足實(shí)驗(yàn)室常規(guī)科研場景下的高效檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD antigen CD50
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縮寫：ICAM3
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma,tissue homogenates
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檢測范圍：31.25 pg/ml - 2000 pg/ml
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靈敏度：7.8 pg/ml
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
225.927
236.470
246.378
218.347
231.580
242.642
SD
0.036
0.044
0.031
0.036
0.046
0.029
CV(%)
4.250
5.052
3.865
4.311
5.336
3.532
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-3/CD50 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
95
Range %
89-100
1:2
Average %
88
Range %
84-92
1:4
Average %
103
Range %
98-107
1:8
Average %
93
Range %
89-97
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回收率：
The recovery of human ICAM-3/CD50 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
84
80-88
EDTA plasma (n=4)
96
91-100
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2000
2.752
2.654
2.703
2.548
1000
1.981
2.015
1.998
1.843
500
1.407
1.386
1.397
1.242
250
0.846
0.872
0.859
0.704
125
0.529
0.508
0.519
0.364
62.5
0.318
0.321
0.320
0.165
31.25
0.235
0.229
0.232
0.077
0
0.153
0.157
0.155
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數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價(jià)
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靶點(diǎn)詳情
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最新研究進(jìn)展：ICAM3又稱為白細(xì)胞共同抗原LFA-1，是一種細(xì)胞表面分子，屬于免疫球蛋白超家族，主要在白細(xì)胞、淋巴細(xì)胞、巨噬細(xì)胞等免疫細(xì)胞表面表達(dá)。ICAM3參與調(diào)節(jié)免疫細(xì)胞之間的黏附作用，在細(xì)胞介導(dǎo)免疫應(yīng)答、免疫細(xì)胞遷移和腫瘤免疫等方面發(fā)揮著重要作用。
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功能：ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages.
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基因功能參考文獻(xiàn)：
- exploration of the underlying mechanism demonstrated that ICAM3 not only binds to LFA-1 with its extracellular domain and structure protein ERM but also to lamellipodia with its intracellular domain which causes a tension that pulls cells apart (metastasis). PMID: 29729315
- Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332
- ICAM3 acts as recognition receptors in the phagocytosis portals of macrophages for engulfment of apoptotic neutrophils. PMID: 23775590
- this data clearly indicate that ICAM-3 promotes drug resistance via inhibition of apoptosis. PMID: 24177012
- Intercellular adhesion molecule (ICAM)-3 mRNA is upregulated in non-adherent endothelial forming cells. PMID: 23144795
- Results indicate that the ICAM-3 gene promoter is negatively regulated by RUNX3. PMID: 22479382
- analysis of activated apoptotic cells induce dendritic cell maturation via engagement of Toll-like receptor 4 (TLR4), dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin (DC-SIGN), and beta2 integrins PMID: 22396536
- ICAM-3 may be an important adhesion molecule involved in chemotaxis to apoptotic human leukocytes. PMID: 22117198
- the molecular basis of allergen-induced Th2 cell polarization PMID: 22205703
- the cross-talk between neutrophils and NK cells is mediated by ICAM-3 and CD11d/CD18, respectively. PMID: 21712539
- Single nucleotide polymorphisms in ICAM3 gene is associated with lymphoma. PMID: 21239057
- induction of morphological polarization in primary T lymphocytes and Jurkat cells enhances Kidins220/ARMS colocalization with ICAM-3 PMID: 21381019
- CCR1 antagonist, BX471, did not significantly alter ICAM-3 expression in relapsing-remitting multiple sclerosis patients. PMID: 20086017
- ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB PMID: 19956847
- no significant risk association was found for SARS infection for the ICAM-3 Asp143Gly SNP. PMID: 19801714
- A novel serine-rich motif in the intercellular adhesion molecule 3 is critical for its ezrin/radixin/moesin-directed subcellular targeting PMID: 11784723
- identification of DC-SIGN binding sites PMID: 11799126
- interactions with DC-SIGN does not promote DC-SIGN mediated HIV-1 transmission PMID: 12021323
- Expression of DC-SIGN and its ligand, ICAM-3, is found in substantial amounts only in RA synovium, suggesting that their interaction is implicated in the additional activation of synovial macrophages that leads to the production of EMMPRIN and MMP-1. PMID: 12571844
- ICAM-3 is highly expressed on the surface of human eosinophils and has a role in the downregulation of GM-CSF production. PMID: 12743567
- Relationship of intercellular adhesion molecule-3 and hepatocyte growth factor with amyloidosis A in chronic renal-failure patients. There was a higher density of intercellular adhesion molecule-3-positive cells in the patients with amyloidosis A. PMID: 14704632
- ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via reactive oxygen species-dependent signaling. PMID: 14726630
- acts as a costimulating molecule to increase HIV-1 transcription and viral replication, a process allowing productive infection of quiescent CD4+ T lymphocytes. PMID: 15163761
- Expression of ICAM-3 can be used as a valuable biomarker to predict the radiation resistance in cervical cancer that occurs during radiotherapy. PMID: 15880373
- The results suggest that ICAM-3 assists in the interaction of granulocytes with DC-SIGN of dendritic cells. PMID: 17145745
- Patients with SARS homozygous for ICAM3 Gly143 showed significant association with higher lactate dehydrogenase levels and lower total white blood cell counts. PMID: 17570115
- This is the first case of CD20 positive mycosis fungoides involving a lymph node to be reported in the literature. PMID: 18261116
- extended alpha(L)beta(2) with an open headpiece is required for ICAM-3 adhesion PMID: 18354203
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亞細(xì)胞定位：Membrane; Single-pass type I membrane protein.
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蛋白家族：Immunoglobulin superfamily, ICAM family
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組織特異性：Leukocytes.
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