Dog Interferon γ,IFN-γ ELISA Kit

Dog Interferon γ,IFN-γ ELISA Kit

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中文名稱：

犬γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E11262c
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
促銷：
其他：

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產(chǎn)品描述：

犬γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E11262c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ，是一種具有多種生物學(xué)功能的細(xì)胞因子。它在免疫調(diào)節(jié)、抗病毒和抗腫瘤等方面發(fā)揮重要作用。其研究機(jī)制涉及激活免疫細(xì)胞、調(diào)控炎癥反應(yīng)、誘導(dǎo)細(xì)胞凋亡等，為多種疾病的治療提供了新的靶點(diǎn)和思路。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，適用于科研場(chǎng)景中犬類免疫相關(guān)研究，例如評(píng)估感染性疾病模型中的免疫應(yīng)答強(qiáng)度、監(jiān)測(cè)疫苗免疫效果、探究炎癥性疾病機(jī)制或分析免疫調(diào)節(jié)藥物的作用效果。為犬類免疫學(xué)、獸醫(yī)學(xué)研究及生物制劑開(kāi)發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
縮寫(xiě)：

IFNG
Uniprot No.：

P42161
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-104
1:2	Average %	96
	Range %	92-102
1:4	Average %	95
	Range %	89-102
1:8	Average %	97
	Range %	90-101

回收率：

The recovery of dog IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-100
EDTA plasma (n=4)	96	89-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.885	1.853	1.869	1.766
100	1.226	1.308	1.267	1.164
50	0.891	0.924	0.908	0.805
25	0.636	0.686	0.661	0.558
12.5	0.407	0.425	0.416	0.313
6.25	0.310	0.318	0.314	0.211
3.12	0.248	0.252	0.250	0.147
0	0.102	0.104	0.103

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
基因功能參考文獻(xiàn)：
1. IL-17A gene transcription level was positively correlated with mRNA expression for iNOS and IFN-gamma PMID: 26590047
2. In dogs with leishmaniasis, there was high expression of interferon gamma in lymph nodes. PMID: 21511273
3. These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917
亞細(xì)胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
組織特異性：

Released primarily from activated T lymphocytes.
數(shù)據(jù)庫(kù)鏈接：

KEGG: cfa:403801

STRING: 9615.ENSCAFP00000000588

UniGene: Cfa.3900

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.