PRKN
PRKN，全名為parkin RBR E3泛素蛋白連接酶，也被稱為帕金蛋白。該蛋白是細胞內重要的“質檢員”，通過識別并標記異常蛋白質，引導細胞清理系統清除受損組分，維持細胞內環境的穩定。PRKN蛋白參與多個生理過程，包括細胞對氨基酸刺激的響應、神經元死亡的負調控以及突觸傳遞的負調控。在神經元死亡負調控的上游或內部發揮作用，位于膜性器官的邊界膜、膜筏以及突觸后密度等細胞組件中，并與線粒體共定位。PRKN蛋白的研究對于理解帕金森病、2型糖尿病等疾病具有重要意義，其人類同源物與帕金森病、帕金森病2和卵巢癌有關。目前，針對PRKN蛋白的藥物研發尚處于早期階段，但已有研究表明，PRKN蛋白的異常可能與帕金森病的發生發展密切相關。
熱銷產品
Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) (CSB-BP017451HU)
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
PRKN Recombinant Monoclonal Antibody (CSB-RA199844A0HU)
驗證數據
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30μg), U-251MG whole cell lysate(30μg), Jurkat whole cell lysate(30μg), K562 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), HEK293T whole cell lysate(30μg), SW620 whole cell lysate(30μg)
All lanes: Parkin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 30 kDa
Exposure time: 2min
Immunofluorescence staining of A-549 cell with CSB-RA199844A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing 786-O cells stained with CSB-RA199844A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
PRKN Antibody (CSB-PA017451LA01HU)
驗證數據
Western Blot
Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate
All lanes: PRKN antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 49, 24, 31, 43, 36, 44, 47 kDa
Observed band size: 52 kDa
IHC image of CSB-PA017451LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA017451LA01HU diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescent analysis of PC-3 cells using CSB-PA017451LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
PRKN Antibodies
PRKN for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA017451LA01HU | PRKN Antibody | Human | ELISA, WB, IHC, IF |
| CSB-PA076233 | PARK2 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA003708 | PARK2 Antibody | Human | WB, IHC, ELISA |
| CSB-PA020235 | Phospho-PARK2 (S131) Antibody | Human | WB, IHC, ELISA |
| CSB-PA020236 | PARK2 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
| CSB-RA199844A0HU | PRKN Recombinant Monoclonal Antibody | Human | ELISA, WB, IF, FC |
PRKN Proteins
PRKN Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP017451HU CSB-MP017451HU CSB-EP017451HU-B |
Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | Yeast Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP017451HU | Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | E.coli |
| CSB-BP017451HU | Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | Baculovirus |
| CSB-BP017451HUb1 | Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) | Baculovirus |
PRKN Proteins for Rattus norvegicus (Rat)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP864348RA CSB-EP864348RA CSB-MP864348RA CSB-EP864348RA-B |
Recombinant Rat E3 ubiquitin-protein ligase parkin (Park2) | Yeast E.coli Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP864348RAb1 | Recombinant Rat E3 ubiquitin-protein ligase parkin (Park2) | E.coli |
| CSB-BP864348RA | Recombinant Rat E3 ubiquitin-protein ligase parkin (Prkn) | Baculovirus |
PRKN Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP893911MO CSB-BP893911MO CSB-MP893911MO CSB-EP893911MO-B |
Recombinant Mouse E3 ubiquitin-protein ligase parkin (Prkn) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
PRKN Proteins for Mus musculus(Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-EP893911MO | Recombinant Mouse E3 ubiquitin-protein ligase parkin (Prkn) | E.coli |
