PIK3CA
PIK3CA，即磷脂酰肌醇4,5-二磷酸3-激酶催化亞基α，別名PI3K-alpha。PIK3CA由人類PIK3CA基因編碼，該基因位于3號染色體的q26.3區域，包含21個外顯子，編碼1068種氨基酸，產生一組長124kD的蛋白。PIK3CA的作用機制涉及催化膜脂磷脂酰肌醇-4,5-二磷酸[PI(4,5)P2]到第二信使PI(3,4,5)P3（也被稱為PIP3）的轉化，激活AKT-mTOR信號通路，促進細胞存活、增殖、生長和運動。PIK3CA是癌癥中最常見的突變基因之一，其激活突變在乳腺癌、子宮內膜癌、膀胱癌、結腸直腸癌等多種癌癥中均有發現，參與腫瘤發生、進展及轉移定植。PIK3CA基因的突變導致PI3K/AKT通路水平上調，進而參與腫瘤發生。
熱銷產品
驗證數據
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
PIK3CA Recombinant Monoclonal Antibody (CSB-RA578819A0HU)
驗證數據
Western Blot
Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate
All lanes: PIK3CA antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 125 kDa
Observed band size: 110 kDa
Immunofluorescence staining of Hela Cells with CSB-RA578819A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
PIK3CA Antibodies
PIK3CA for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA017998GA01HU | PIK3CA Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA003761 | PIK3CA Antibody | Human,Mouse | WB, IHC, ELISA |
| CSB-PA017998LA01HU | PIK3CA Antibody | Human | ELISA, IHC, IF |
| CSB-PA017998LC01HU | PIK3CA Antibody, FITC conjugated | Human | |
| CSB-PA017998LD01HU | PIK3CA Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA578819A0HU | PIK3CA Recombinant Monoclonal Antibody | Human, Mouse | ELISA, WB, IF |
PIK3CA Proteins
PIK3CA Proteins for Bos taurus (Bovine)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP017998BO CSB-EP017998BO CSB-BP017998BO CSB-MP017998BO CSB-EP017998BO-B |
Recombinant Bovine Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
PIK3CA Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP017998HU CSB-BP017998HU CSB-MP017998HU CSB-EP017998HU-B |
Recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP017998HU | Recombinant Human?Phosphatidylinositol?4,5-bisphosphate?3-kinase?catalytic?subunit?alpha?isoform (PIK3CA), partial | E.coli |
PIK3CA Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP017998MO CSB-EP017998MO CSB-BP017998MO CSB-MP017998MO CSB-EP017998MO-B |
Recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (Pik3ca), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
PIK3CA ELISA Kit
PIK3CA ELISA Kit for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-E08417h | Human Phosphotylinosital 3 kinase,PI3K ELISA Kit | serum, tissue homogenates, cell lysates | 0.156 ng/mL |
PIK3CA ELISA Kit for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-E08419m | Mouse Phosphotylinosital 3 kinase,PI3K ELISA Kit | serum, plasma, tissue homogenates | 5.8 pg/mL |
