IRAK4
IRAK4，即白細胞介素-1受體相關激酶4，別名IL-1受體相關激酶4。它是一種在Toll樣受體（TLRs）和IL-1受體介導的炎癥發生中的關鍵性蛋白。IRAK4由人體細胞自身的基因編碼，其作用機制主要是通過參與Toll樣受體信號通路，激活下游的NF-κB和AP-1轉錄因子，從而誘導炎癥因子、細胞因子、趨化因子的表達 。IRAK4在生物學上的意義非常重要，它不僅參與了炎癥反應的正向調節，還廣泛參與了細胞內反應的網絡性調節。IRAK4的功能異常與多種炎癥性疾病的發生發展有關，包括類風濕性關節炎、特應性皮炎、化膿性汗腺炎等。此外，IRAK4的過表達可以增強LPS誘導的NADPH氧化酶活性，誘導下游的p38 MAPK的信號轉導，進一步擴展了IRAK4的生物學功能 。因此，IRAK4是抗炎癥和腫瘤研究中的一個重要靶點，針對IRAK4的小分子抑制劑和蛋白降解劑正在被開發用于治療相關疾病。
熱銷產品
IRAK4 Recombinant Monoclonal Antibody (CSB-RA284992A0HU)
驗證數據
Western Blot
Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate
All lanes: IRAK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 38 kDa
Observed band size: 55 kDa
Overlay histogram showing Jurkat cells stained with CSB-RA284992A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
IRAK4 Antibody (CSB-PA011812ESR1HU)
驗證數據
Western blot
All lanes: Interleukin-1 receptor-associated kinase 4 antibody at 4μg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52, 38 kDa
Observed band size: 52 kDa
Immunoprecipitating IRAK4 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA011812ESR1HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA011812ESR1HU (8μg) + MCF-7 whole cell lysate (500μg)
Lane 3: MCF-7 whole cell lysate (10μg)
IRAK4 Antibodies
IRAK4 for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA011812ESR1HU | IRAK4 Antibody | Human | ELISA, WB, IP |
| CSB-PA011812ESR2HU | IRAK4 Antibody | Human | ELISA |
| CSB-PA793788 | IRAK4 Antibody | Human,Mouse | ELISA,IHC |
| CSB-RA284992A0HU | IRAK4 Recombinant Monoclonal Antibody | Human | ELISA, WB, FC |
IRAK4 Proteins
IRAK4 Proteins for Mus musculus (Mouse)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP011812MO CSB-EP011812MO CSB-BP011812MO CSB-MP011812MO CSB-EP011812MO-B |
Recombinant Mouse Interleukin-1 receptor-associated kinase 4 (Irak4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IRAK4 Proteins for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP011812HU CSB-EP011812HU CSB-BP011812HU CSB-MP011812HU CSB-EP011812HU-B |
Recombinant Human Interleukin-1 receptor-associated kinase 4 (IRAK4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IRAK4 Proteins for Bos taurus (Bovine)
| 產品貨號 | 產品名稱 | 來源 |
|---|---|---|
| CSB-YP011812BO CSB-EP011812BO CSB-BP011812BO CSB-MP011812BO CSB-EP011812BO-B |
Recombinant Bovine Interleukin-1 receptor-associated kinase 4 (IRAK4) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
IRAK4 ELISA Kit
IRAK4 ELISA Kit for Homo sapiens (Human)
| 產品貨號 | 產品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-E16338h | Human interleukin-1 receptor-associated kinase 4(IRAK-4)ELISA Kit | serum, plasma, tissue homogenates | 1.56 pg/mL |
