ATP7B

ATP7B(ATPase copper transporting beta)，是一種跨膜ATP酶蛋白。它在細胞內(nèi)負責(zé)調(diào)節(jié)銅元素的轉(zhuǎn)運和代謝。ATP7B的結(jié)構(gòu)包括多個功能區(qū)域，其中包括多個轉(zhuǎn)運結(jié)構(gòu)域和催化ATP水解的結(jié)構(gòu)域。這些結(jié)構(gòu)域協(xié)同作用，使得ATP7B能夠在細胞膜上運輸銅離子。 ATP7B主要表達在肝臟細胞的高銅含量亞細胞器中，如高銅含量的高爾基體和胞質(zhì)溶酶體。它參與了細胞內(nèi)銅離子的轉(zhuǎn)運和分配，將銅從細胞質(zhì)輸送到高爾基體或從高爾基體釋放到胞質(zhì)溶酶體。 ATP7B的功能對于維持體內(nèi)銅平衡和避免銅積累至毒性水平非常重要。銅是生命過程中的關(guān)鍵元素，但在高濃度下會對細胞和組織產(chǎn)生毒性作用。因此，ATP7B的異常功能或突變可能導(dǎo)致Wilsons病，這是一種遺傳性銅代謝紊亂疾病。對ATP7B的研究對于理解銅代謝的調(diào)控機制以及Wilsons病的發(fā)病機理非常重要。同時，ATP7B也成為了治療Wilsons病的一個潛在靶點，研發(fā)針對ATP7B的治療方法有望改善這種疾病的治療效果。

熱銷產(chǎn)品

● ATP7B Recombinant Monoclonal Antibody CSB-RA175460A0HU

驗證數(shù)據(jù)

IHC

IHC image of CSB-RA175460A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB

IHC

IHC image of CSB-RA175460A0HU diluted at 1:50 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB

Immunofluorescence staining of HepG2 with CSB-RA175460A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 494-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing HepG2 cells stained with CSB-RA175460A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ATP7B Antibody

ATP7B Protein

ATP7B Antibodies

ATP7B for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA002415ESR2HU	ATP7B Antibody	Human	ELISA, IHC
CSB-PA030103	ATP7B Antibody	Human,Mouse,Rat	IHC, IF, ELISA
CSB-RA175460A0HU	ATP7B Recombinant Monoclonal Antibody	Human	ELISA, IHC, IF, FC
CSB-RA002415MA1HU	ATP7B Recombinant Monoclonal Antibody	Human	ELISA, IF, FC

ATP7B Proteins

ATP7B Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP002415HU CSB-BP002415HU CSB-MP002415HU CSB-EP002415HU-B	Recombinant Human Copper-transporting ATPase 2 (ATP7B), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP002415HU	Recombinant Human Copper-transporting ATPase 2 (ATP7B), partial	E.coli

ATP7B Proteins for Rattus norvegicus (Rat)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP714496RA CSB-EP714496RA CSB-BP714496RA CSB-MP714496RA CSB-EP714496RA-B	Recombinant Rat Copper-transporting ATPase 2 (Atp7b), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

ATP7B Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP723743MO CSB-EP723743MO CSB-BP723743MO CSB-MP723743MO CSB-EP723743MO-B	Recombinant Mouse Copper-transporting ATPase 2 (Atp7b), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

ATP7B Proteins for Ovis aries (Sheep)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP896432SH CSB-EP896432SH CSB-BP896432SH CSB-MP896432SH CSB-EP896432SH-B	Recombinant Sheep Copper-transporting ATPase 2 (ATP7B), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli