Recombinant Mouse Treacle protein (Tcof1), partial

中文名稱：

小鼠Tcof1重組蛋白
貨號：

CSB-YP023319MO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Tcof1重組蛋白
貨號：

CSB-EP023319MO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Tcof1重組蛋白
貨號：

CSB-EP023319MO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Tcof1重組蛋白
貨號：

CSB-BP023319MO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Tcof1重組蛋白
貨號：

CSB-MP023319MO
規(guī)格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Tcof1
Uniprot No.：

O08784
別名：

Tcof1Treacle protein; Treacher Collins syndrome protein homolog
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.
基因功能參考文獻：
1. Role of Tcof1 in normal embryonic development, the correlation between genetic and environmental factors on the severity of craniofacial abnormalities, and the prospect for prenatal prevention of craniofacial anomalies. [review] PMID: 27481486
2. Tcof1 acts as a modifier of Pax3 during enteric nervous system development. PMID: 23283078
3. identified Tcof1 as an important regulator of vagal neural crest cells (NCC) development and enteric nervous system formation; Tcof1 loss-of-function results in a deficiency of vagal NCC and their delayed colonization of the gut during early embryogenesis, which mimics the early stages of Hirschsprung's disease PMID: 22228097
4. our research has therefore identified Treacle and as novel in vivo regulators of spindle fidelity, mitotic progression, and proliferation in the maintenance and localization of neural progenitor cells. PMID: 22479190
5. Loss-of-function mutation in Tcof1 results in defects in middle ear postnatal development and conductive hearing loss. PMID: 20106873
6. Results show that treacle is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). PMID: 15249688
7. Data show that Tcof1 and treacle synthesis play an important role in the proliferation of neuroblastoma cells. PMID: 15522210
8. Analysis of RNA isolated from wild-type and Tcof1+/- heterozygous mice embryos from strains that exhibit a lethal phenotype showed significant reduction in 2'-O-methylation at nucleotide C463 of 18S rRNA PMID: 15930015
9. A minimal promoter fragment from -253 to +43 bp directs constitutive expression in both cell types, and dual regulation of Tcof1 appears to be through differential repression of this minimal promoter. PMID: 18771418
10. It has been hypothesized that mutations in Tcof1 disrupt ribosome biogenesis to a degree that is insufficient to meet the proliferative needs of the neuroepithelium and neural crest cells. PMID: 19027870
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亞細胞定位：

Nucleus, nucleolus.
組織特異性：

Ubiquitous in adult and embryonic tissues.
數(shù)據(jù)庫鏈接：

KEGG: mmu:21453

STRING: 10090.ENSMUSP00000135639

UniGene: Mm.486251