Recombinant Mouse Telomerase reverse transcriptase (Tert), partial

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中文名稱：

小鼠Tert重組蛋白
品名簡稱：

Recombinant Mouse Tert protein, partial
貨號：

CSB-EP023391MO
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Tert
Uniprot No.：

O70372
別名：

Tert; Telomerase reverse transcriptase; EC 2.7.7.49; Telomerase catalytic subunit
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

54.1kDa
表達區域：

595-928aa
氨基酸序列

EVRHHQDTWLAMPICRLRFIPKPNGLRPIVNMSYSMGTRALGRRKQAQHFTQRLKTLFSMLNYERTKHPHLMGSSVLGMNDIYRTWRAFVLRVRALDQTPRMYFVKADVTGAYDAIPQGKLVEVVANMIRHSESTYCIRQYAVVRRDSQGQVHKSFRRQVTTLSDLQPYMGQFLKHLQDSDASALRNSVVIEQSISMNESSSSLFDFFLHFLRHSVVKIGDRCYTQCQGIPQGSSLSTLLCSLCFGDMENKLFAEVQRDGLLLRFVDDFLLVTPHLDQAKTFLSTLVHGVPEYGCMINLQKTVVNFPVEPGTLGGAAPYQLPAHCLFPWCGLLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
基因功能參考文獻：
1. Par-4 activation and binding to TERT are key steps required for inducing the apoptosis of islet beta cells under high-glucose/fatty acid conditions in type 2 diabetes. PMID: 30186877
2. Tert-deficient but not Terc-deficient mice develop hepatocyte injury and frank steatosis when challenged with liquid high-fat diet. PMID: 28741793
3. identification of a subset of hepatocytes that expresses high levels of telomerase and show that this hepatocyte subset repopulates the liver during homeostasis and injury PMID: 29618815
4. These preclinical data in mouse models and human cells provide a strong rationale for the development of pharmacological approaches to target BMI1-mediated mitochondrial regulation and protection from DNA damage to sustain the regenerative potential of the skeletal muscle in conditions of chronic muscle wasting. PMID: 28757168
5. Reactivation of Tert in the hippocampus was sufficient to normalize the depressive but not the aggressive behaviors of Tert(-/-) mice. Conversely, re-expression of Tert in the medial prefrontal cortex (mPFC) reversed the aggressive but not the depressive behavior of Tert(-/-) mice. PMID: 27300262
6. Nrf2-driven TERT regulates pentose phosphate pathway in glioblastoma. PMID: 27148686
7. TERT has a role in neointima formation through epigenetic regulation of proliferative E2F1 target gene expression in smooth muscle cells. PMID: 27932351
8. these findings support a model in which gain of TERT function modulates mTORC1 activity and induces autophagy. PMID: 27545609
9. Regarding extratelomeric activities, our results showed a decrease of 64, 38 and 25% in the transcription of c-Myc, Cyc-D1 and TERT, respectively (p<0.05) after AZT treatment. Furthermore, we found an effect on cell migration, reaching an inhibition of 48% (p<0.05) and a significant passage-dependent increase on cell doubling time during treatment PMID: 27633795
10. Results suggest that in mature Purkinje neurons, TERT is present both in the nucleus and in mitochondria, where it may participate in adaptive responses of the neurons to excitotoxic and radiation stress PMID: 26374457
11. Wnt10a/beta-catenin signaling pathway is able to exacerbate keloid cell proliferation and inhibit the apoptosis of keloid cells through its interaction with TERT. PMID: 27771714
12. This study reports the characterisation of two novel mouse TERT splice variants, Ins-i1[1-102] (Insi1 for short) and Del-e12[1-40] (Dele12 for short) that have not been previously described. Insi1 represents an in-frame insertion of nucleotides 1-102 from intron 1, encoding a 34 amino acid insertion at amino acid 73. PMID: 26787169
13. TERT may promote gastric cancer metastasis through the TERT-miR-29a-ITGB1 regulatory pathway. PMID: 26903137
14. TERT switches macrophages towards M1 phenotype by regulating NF-kappaB signaling, but has limited effect on M2 macrophages polarization in vitro. PMID: 26725521
15. The results suggest that the mouse endometrial epithelium and vasculature are foci of stem/progenitor activity expressing mTert. PMID: 26740067
16. miR-195 overexpressed in old mesenchymal stem cells (OMSCs) induces stem cell senescence deteriorating their regenerative ability by directly deactivating telomerase reverse transcriptase (Tert), and abrogation of miR-195 can reverse stem cell aging. PMID: 26390028
17. These findings indicate that telomerase gene therapy represents a novel therapeutic strategy to treat aplastic anemia provoked or associated with short telomeres. PMID: 26903545
18. findings identified a key role for TERT in fibroblast proliferation and survival essential for pulmonary fibrosis PMID: 26555817
19. miR-512-5p suppresses tumor growth by targeting TERT in telomerase positive head and neck squamous cell carcinoma in vitro and in vivo. PMID: 26258591
20. Telomerase deficiency triggers alveolar stem cell replicative senescence-associated low-grade inflammation. PMID: 26518879
21. high telomerase expression is a fundamental characteristic of germline stem cells. PMID: 26584619
22. data suggest that S1P binding to hTERT allosterically mimicks phosphorylation, promoting telomerase stability and hence telomere maintenance, cell proliferation, and tumor growth. PMID: 26082434
23. Telomerase may direct Pol I transcription in oncogenic and regenerative hyperproliferation. PMID: 25118183
24. Tert expression confers cardioprotection in the adult mouse heart after acute myocardial infarction. PMID: 25519492
25. TERT is a regulator of MYC stability in cancer. Reactivation of TERT, a direct transcriptional MYC target in tumors, provides a feed-forward mechanism to potentiate MYC-dependent oncogenesis. PMID: 25893605
26. Pharmacologically relevant doses of atorvastatin resulted in a 6-fold increase of telomerase activity in mouse PBMCs and CD4 T cells. Transgenic GFP-mTert reporter mice had 30% to 15% less telomerase-positive lymphocytes during the first 5 months of age. PMID: 25127175
27. Studies indicate that reverse transcriptase (RT) enzyme highly expressed in mouse embryos and mouse and human cancer cells and repressed in somatic differentiated healthy cells. PMID: 25586649
28. The overexpression of Zfp637 markedly increases mTERT expression and telomerase activity, maintains telomere length, and inhibits H2O2 and D-galactose-induced senescence. PMID: 25032857
29. Telomerase exerts telomere-independent effects on pulmonary artery smooth muscle cell growth in pulmonary hypertension. PMID: 25550449
30. These findings establish a functional link between endoplasmic reticulum stress and telomerase. PMID: 24119029
31. TERT, combined with beta-catenin and BRG1, serves as a transcriptional complex, which binds the FAS ligand (FASL) promoter to upregulate FASL expression, leading to an elevated immunomodulatory function. PMID: 24401839
32. These data indicate that TERT plays an extratelomeric role in the reprogramming process, but its function is dispensable. PMID: 24733392
33. Once HIV production had reached a peak (7 dpi), the telomerase activity decreased, showing levels similar to those of noninfected cells PMID: 24254728
34. Overexpression of TERT in mesenchmal stem cells resulted in increased cell proliferation. PMID: 22884695
35. TERT expression was up-regulated by a histone deacetylase inhibitor, while the induction of TERT in lung fibroblasts was associated with the binding of acetylated histone H3K9 to the TERT promoter region. PMID: 23526226
36. calorie restriction attenuates telomere erosion associated to aging and that synergizes with TERT over-expression in increasing "health span" and extending mouse longevity PMID: 23349740
37. prepared two mutant forms of the PhSurv-PhTERT tandem with two or four Sp1 sites removed from the distal "long" PhSurv promoter PMID: 23056318
38. Study identified the cells responsible for cardiac telomerase activity, demonstrates a significant diminution with age. PMID: 22919071
39. mir498 has a role in 1,25-Dihydroxyvitamin D3 suppression of telomerase expression and cancer growth PMID: 23055531
40. Data show that AUF1 binds and strongly activates the transcription promoter for telomerase catalytic subunit Tert. PMID: 22633954
41. Essential role for telomerase in chronic myeloid leukemia induced by BCR-ABL in mice. PMID: 22408137
42. telomerase plays a role in the aging of nondividing cells PMID: 22533433
43. findings show beta-catenin regulates Tert expression through interaction with Klf4, a core component of the pluripotency transcriptional network; beta-Catenin binds to the Tert promoter in a mouse intestinal tumor model PMID: 22723415
44. Data provide evidence that telomere dysfunction plays a critical role in prostate cancer initiation and progression, permitting acquisition of and selection for cancer-relevant genomic events upon telomerase reactivation. PMID: 22341455
45. Silencing transgenic TERT expression or inhibiting Wnt signaling through systemic expression of the Wnt inhibitor Dkk1 in either TERT transgenic mice or in a mouse model of HIVAN results in marked normalization of podocytes PMID: 22138751
46. Selenium and benzene can upregulate the telomerase activity in mouse lymphocytes in vivo. PMID: 19080380
47. Hippocampal telomerase is involved in the modulation of depression-related behaviors, possibly by regulating adult neurogenesis. PMID: 21865469
48. Primitive hematopoietic Tert(-/-) cells lacking telomerase activity exhibit signs of enhanced DNA damage. PMID: 21730353
49. Results indicate that both telomerase reverse transcriptase and telomerase RNA are haploinsufficient and that their deficiency leads to telomere shortening, which limits tissue renewal. PMID: 21464209
50. The results of this study provide the first experimental evidence that telomere shortening, despite impairing adult neurogenesis and maintenance of post-mitotic neurons, can slow down the progression of amyloid plaque pathology in Alzheimer's disease. PMID: 21672962
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亞細胞定位：

Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Chromosome, telomere. Cytoplasm. Nucleus, PML body.
蛋白家族：

Reverse transcriptase family, Telomerase subfamily
組織特異性：

High activity in intestine, liver and testis, moderate in lung, very low in muscle, heart and brain.
數據庫鏈接：

KEGG: mmu:21752

STRING: 10090.ENSMUSP00000022104

UniGene: Mm.10109