Recombinant Mouse Probable phospholipid-transporting ATPase IC (Atp8b1), partial

中文名稱：

Recombinant Mouse Probable phospholipid-transporting ATPase IC(Atp8b1) ,partial
貨號：

CSB-YP614816MO
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Probable phospholipid-transporting ATPase IC(Atp8b1) ,partial
貨號：

CSB-EP614816MO
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse Probable phospholipid-transporting ATPase IC(Atp8b1) ,partial
貨號：

CSB-EP614816MO-B
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Probable phospholipid-transporting ATPase IC(Atp8b1) ,partial
貨號：

CSB-BP614816MO
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Probable phospholipid-transporting ATPase IC(Atp8b1) ,partial
貨號：

CSB-MP614816MO
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Atp8b1
Uniprot No.：

Q148W0
別名：

Atp8b1Phospholipid-transporting ATPase IC; EC 7.6.2.1; ATPase class I type 8B member 1; P4-ATPase flippase complex alpha subunit ATP8B1
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of phospholipids, in particular phosphatidylcholines (PC), from the outer to the inner leaflet of the plasma membrane. May participate in the establishment of the canalicular membrane integrity by ensuring asymmetric distribution of phospholipids in the canicular membrane. Thus may have a role in the regulation of bile acids transport into the canaliculus, uptake of bile acids from intestinal contents into intestinal mucosa or both and protect hepatocytes from bile salts. Involved in the microvillus formation in polarized epithelial cells; the function seems to be independent from its flippase activity. Participates in correct apical membrane localization of CDC42, CFTR and SLC10A2. Enables CDC42 clustering at the apical membrane during enterocyte polarization through the interaction between CDC42 polybasic region and negatively charged membrane lipids provided by ATP8B1. Together with TMEM30A is involved in uptake of the synthetic drug alkylphospholipid perifosine. Required for the preservation of cochlear hair cells in the inner ear. According PubMed:20852622 is proposed to act as cardiolipin transporter during inflammatory injury; the function is questioned by PubMed:21475228.
基因功能參考文獻(xiàn)：
1. ATP8B1 is important for proper CFTR expression and function. PMID: 27301931
2. to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. PMID: 26416959
3. Flippase ATP8B1 and the floppase ABCB4 have complementary functions in maintaining canalicular membrane integrity. PMID: 21820390
4. results unveil a new paradigm whereby Atp8b1 is a cardiolipin importer whose capacity to remove cardiolipin from lung fluid is exceeded during inflammation or when Atp8b1 is defective PMID: 20852622
5. Data show that ATP8B1-deficient pups of B6 background gained less weight. PMID: 20126555
6. mutation disrupts bile salt homeostasis without impairment of canalicular bile secretion PMID: 14976163
7. Fic1 is expressed in a tissue-specific and developmentally regulated fashion at the apical membranes of epithelial cells PMID: 15496606
8. Atp8b1 deficiency causes loss of canalicular phospholipid membrane asymmetry that in turn renders the canalicular membrane less resistant toward hydrophobic bile salts. PMID: 16799980
9. The accumulation of bile salt (BS) in plasma of Atp8b1(G308V/G308V) mice during BS feeding is not caused by increased intestinal BS absorption. PMID: 17448567
10. show that ATP8B1/Atp8b1 deficiency, both in patients and in Atp8b1(G308V/G308V) mutant mice, causes hearing loss, associated with progressive degeneration of cochlear hair cells PMID: 19478059
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亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein. Apical cell membrane. Cell projection, stereocilium. Endoplasmic reticulum. Golgi apparatus.
蛋白家族：

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IV subfamily
組織特異性：

Hepatocytes, bile duct, intestinal epithelial cells (cholangiocytes and ileocytes), and pancreatic acinar cells.
數(shù)據(jù)庫鏈接：

KEGG: mmu:54670

STRING: 10090.ENSMUSP00000025482

UniGene: Mm.270043