Recombinant Mouse Polycystin-2 (Pkd2), partial
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中文名稱：小鼠Pkd2重組蛋白
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貨號：CSB-EP018061MO-B
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說明書：
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規(guī)格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產(chǎn)品詳情
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純度：>85% (SDS-PAGE)
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基因名：Pkd2
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Uniprot No.：
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別名：Pkd2; TRPP2; Polycystin-2; Polycystic kidney disease 2 protein homolog; Transient receptor potential cation channel subfamily P member 2
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種屬：Mus musculus (Mouse)
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蛋白長度：Partial
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蛋白標(biāo)簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產(chǎn)品評價
靶點詳情
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功能：Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B. Can also form a functional, homotetrameric ion channel. Functions as a cation channel involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Functions as outward-rectifying K(+) channel, but is also permeable to Ca(2+), and to a much lesser degree also to Na(+). May contribute to the release of Ca(2+) stores from the endoplasmic reticulum. Together with TRPV4, forms mechano- and thermosensitive channels in cilium. PKD1 and PKD2 may function through a common signaling pathway that is necessary to maintain the normal, differentiated state of renal tubule cells. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Also involved in left-right axis specification via its role in sensing nodal flow; forms a complex with PKD1L1 in cilia to facilitate flow detection in left-right patterning. Detection of asymmetric nodal flow gives rise to a Ca(2+) signal that is required for normal, asymmetric expression of genes involved in the specification of body left-right laterality.
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基因功能參考文獻(xiàn)：
- Pkhd1(Flox67HA) is a valid mouse model of autosomal recessive polycystic kidney disease to track Pkhd1-derived products containing the C-terminus. Significantly, exon 67 containing the nuclear localization signal and the polycystin-2 binding domain is not essential for Fibrocystin function in this model. PMID: 28729032
- Knocking out TRPP2 by CRISPR/Cas9 genome editing eliminated the channel current, establishing it as TRPP2 dependent PMID: 27760766
- olycystin 2 provides a critical function in the heart, independent of renal involvement PMID: 27081851
- The TRPP2 functionally interacts with both ryanodine and InsP3 receptors. These interactions were not similar in adult and old mice. PMID: 26254047
- Pkd2(-/-) mice with homozygous PKD2(tg)-transgene alleles (Pkd2(-/-);PKD2(tg/tg)) showed significant further amelioration of the cystic severity compared to that in Pkd2(-/-) mice PMID: 26435415
- Pkd1 and Pkd2 have coordinate effects on osteoblast differentiation and opposite effects on adipogenesis, suggesting that Pkd1 and Pkd2 signaling pathways can have independent effects on mesenchymal lineage commitment in bone PMID: 25464512
- PKD2 regulates directly and indirectly about 5% of the cytotoxic T-cell phosphoproteome. PMID: 25266776
- In inner medullary collecting duct, flow, via polycystin-2 and P2 receptors, engages Ca(2+)-dependent signaling pathways that stimulate ET-1 synthesis. PMID: 25587122
- Pkd2(+/-) cardiomyocytes shift the beta adrenergic receptor pathway and have altered calcium handling, independent of desensitized calcium-contraction coupling. PMID: 25368166
- Epithelial-specific disruption of Pkd2 disrupts male reproductive tract development. PMID: 24951251
- AGT inhibition resulted in significant decreases in kidney size and cyst volume and an improvement in kidney function in mice with targeted mutation in Pkd2. PMID: 25537744
- an epidermal growth factor activated TRPP2\TRPV4 channel may play an important role in increased cell proliferation and cystogenesis. PMID: 23977387
- Expression of PC2 and PC1 in osteocyte-like cells is increased by oscillatory fluid flow PMID: 24268313
- Pkd2 is likely to play a critical role in craniofacial growth as a mechanoreceptor. Pkd2 is also identified as one of the genes responsible for autosomal dominant polycystic kidney disease (ADPKD). PMID: 23390131
- The defect in vasodilatation in Pkd2 heterozygous vessels is primarily due to a reduction in nitric bioavailability secondary to increased vascular oxidative stress. PMID: 23103499
- these results thus suggest that nodal flow is sensed in a manner dependent on Pkd2 by the cilia of crown cells located at the edge of the node. PMID: 22983710
- Protein kinase D2-deficient lymphocytes bound normally to integrin ligands in static and shear flow adhesion assays. They also homed normally to lymphoid organs after adoptive transfer into wild-type mice. PMID: 22311617
- Study concludes that mechanical injury of brain endothelial cells induces a rapid influx of calcium, mediated by TRPC1 and TRPP2 channels, which leads to NO synthesis and actin cytoskeletal rearrangement. PMID: 22192412
- polycystin-2 forms functional channels in retinal ganglion cells, of which biophysical and pharmacological properties are similar to polycystin-2 channels reported for other tissues PMID: 22155264
- All channel-forming isoforms of TRPP channels (polycystin-2, polycystin-L, and polycystin-2L2) were expressed in adult mouse lacrimal gland. PMID: 21508103
- findings identify PC2 and PDE4C as unique components of an AKAP complex in primary cilia and reveal a common mechanism for dysregulation of cAMP signaling in cystic kidney diseases arising from different gene mutations PMID: 21670265
- identify PKDs, in particular PKD2, as a major mediator of muscle cell differentiation in vitro PMID: 21298052
- Data show that PC-2 regulates FSS-induced MAP kinase trafficking into the nucleus of CD cells. PMID: 21109758
- data suggest that TAZ and Nek1 constitute a negative feedback loop linked through phosphorylation and ubiquitination and that the interaction of Nek1 and TAZ maintain PC2 at the level needed for proper ciliogenesis PMID: 21474562
- Pkd1l1 is the elusive Pkd2 binding partner required for Left-Right patterning. PMID: 21307093
- Male pkd2(WS25/-) mice consistently display a late progression in liver growth PMID: 20388629
- PC-2 may mediate cell-cell adhesion, at least partially, through E-cadherin. PMID: 19688268
- Pkd2ws25/- mice exhibit hepatorenal pathology resembling human autosomal dominant polycystic kidney disease. PMID: 20093497
- Data suggest that Bicc1 functions by modulating the expression of polycystin 2 (Pkd2 by antagonizing the repressive activity of the miR-17 microRNA family on the 3'UTR of Pkd2 mRNA. PMID: 20215348
- In Pkd2-defective liver endothelial cells, PKA-dependent ERK1/2 signaling controls HIF-1alpha-dependent VEGF secretion and VEGFR-2 signaling PMID: 19766642
- TRPP gene is expressed in the mouse inner ear. PMID: 20095091
- functions as a calcium-activated intracellular calcium release channel in vivo, and polycystic kidney disease results from the loss of a regulated intracellular calcium release signalling mechanism PMID: 11854751
- role in left-right axis determination and elucidation that it acts downstream or in parallel to shh and upstream of the nodal cascade PMID: 12062060
- localization to kidney cilia and elevation of level in polycystic kidney disease PMID: 12062067
- PC2 functions as a plasma membrane channel in renal epithelia and may contribute to Ca(2+) entry and transport of other cations in defined nephron segments in vivo PMID: 12640140
- polycystin-2 has a role in regulating the localization of polycystin-1 in mammalian cells PMID: 12840011
- Calcium dependence of polycystin-2 channel activity is modulated by phosphorylation at Ser812 PMID: 14742446
- Pericentrin, intraflagellar transport proteins, and Polycystin-2 form a complex in vertebrate cells that is required for assembly of primary cilia and possibly motile cilia and flagella. PMID: 15337773
- Our results demonstrate a PC1-independent function of PC2 in left-right axis formation, and indirectly support a ciliary role of PC2 in this process. PMID: 16316413
- Proliferating tubular cells were uniformly positive for Pkd2 expression in Pkd2(+/-) kidney. Pkd2 haploinsufficiency may cause an increase in cell proliferation preceding cyst formation. PMID: 16720597
- Non-invasive phenotyping using ultrasound did not reveal significant cardiovascular difference between adult Pkd2(+/LacZ) and WT mice. PMID: 17182135
- arterial vasculature in Pkd2(+/-) mice exhibits an exaggerated contractile response and increased sensitivity to phenylephrine. PMID: 17202419
- These findings suggest that FPC and polycystins share, at least in part, a common mechanotransduction pathway. PMID: 17283055
- PC2 is important for regulation of RyR2 function and that loss of this regulation of RyR2, as occurs when PC2 is mutated, results in altered Ca(2+) signaling in the heart. PMID: 17404231
- overexpression of human PKD2 leads to anomalies in tubular function, probably due to abnormalities in tubule morphogenesis. PMID: 18048422
- TNF-alpha, an inflammatory cytokine, disrupts the localization of polycystin-2 to the plasma membrane and primary cilia through a scaffold protein, FIP2, which is induced by TNF-alpha PMID: 18552856
- Results suggest that polycystin-2 plays a role in centrosome duplication. PMID: 18725310
- PC2 down-regulation is accompanied by a phenotype similar to that of Pkhd1(-/-) mice PMID: 18782757
- Pkd2 in the ER plays a role in cellular Ca(2+) homeostasis and Stx5 functions to inactivate Pkd2 and prevent leaking of Ca(2+) from ER stores PMID: 18836075
- ERK activation was induced by PKD2 overexpression via B-Raf signaling, providing a possible molecular mechanism of cystogenesis PMID: 19098310
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亞細(xì)胞定位：Cell projection, cilium membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane. Endoplasmic reticulum membrane. Golgi apparatus.
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蛋白家族：Polycystin family
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組織特異性：Detected in kidney epithelium (at protein level). Highly expressed on basolateral membranes in distal convoluted tubules and medullary thick ascending limbs of Henle. Detected at much lower levels in cortical and medullary collecting tubules, and not dete
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